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Clear
 
Test Code:
090-10-0351-01

Order Name:
Widal Test

 
Useful For:
Stained antigen suspension are used for the identification and quantitative detection of specific antibodies in human sera for epidemiological and diagnostic purpose, primarily in the investigation of pyrexia and enteric infections with certain Salmonellae, Rickettsiae and Brucellae pathogens.
 
Methodology:
Agglutination
 
AliasesName:
Salmonellae
Rickettsiae
Brucellae 
Typhoid fever
 
 
 
Test Code:
090-10-0351-01

Order Name:
Widal Test

 
Collection Specimen Or Container:
Blood/ Plain Blood (Red Top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 1 mL
 
Sub Mission Container:
Plastic tube
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 48 hours
 
 
 
Test Code:
090-10-0351-01

Order Name:
Widal Test

 
Method detail:
Agglutination
 
Schedule:
Tested Daily (24 hours)
 
Turnaround Time:
Collected specimen to report within 90 mins.
 
Performing Location:
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
090-10-0351-01

Order Name:
Widal Test

 
 
Clinical Information:
Typhoid fever and Salmonellosis are epidermic in many pasts of the developing world. The widal test is the most widely used test to demonstrate Salmonella O and H antigens in the patient serum Its ease of use and the unavailability of microbiological facilities to perform blood culture along with the time taken to confirm results has led to the preference of the widal test. This however has led to physicians placing too much reliance on a single widal test. It is essential follow up test are performed to demonstrate a 2-4 fold increase in titre indicative of current infection
 
Reference Value:
≤ 1:80
 
Interpretation:
If agglutination reaction are observed the following approximate titres would be observed if a confirmatory tube test was carried out : 80 ul. = 1:20 40 ul = 1:40 20 ul = 1:80 10 ul = 1:160 5 ul = 1:320 In this way the rapid slide test provides an approximation to the expected results from a corresponding tube test Note : It is necessary to perform all dilutions in the slides test to obviate the prozone effect where higher concentrations of the serum may give a negative resukt but further dilutions may give a positive result.
 
Clinical Reference:
Manufacturer’s reagent package insert Serosystem. Clinical diagnostic 2003