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Clear
 
Test Code:
090-40-0450-01

Order Name:
Sputum C/S

 
Useful For:
Diagnosis of bacterial infections
 
Methodology:
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
AliasesName:
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
Sputum culture
 
 
 
Test Code:
090-40-0450-01

Order Name:
Sputum C/S

 
Patient Preparation:
Medications: Do not use antibiotics before collection of specimen
  1. Have patient rinse or gargle with water to remove excess oral biota. 
  2. Instruct patient to cough deeply to produce a lower respiratory specimen (not postnasal fluid)
  3. Collect in a sterile container
 
Collection Specimen Or Container:
Sputum/ Sterile container
 
Specimen Testing Type:
Sputum, volume Entire collection (minimum 0.5 mL)
 
Sub Mission Container:
Sterile container
 
Rejection Criteria:
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Sputum Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0450-01

Order Name:
Sputum C/S

 
Method detail:
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to reported within 3 days.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0450-01

Order Name:
Sputum C/S

 
 
Clinical Information:
Common bacterial organisms of acute pneumonia include: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Pseudomonas aeruginosa, and members of the Enterobacteriaceae. Clinical history, physical examination, and chest X-ray are usually adequate for the diagnosis of pneumonia, and antimicrobial treatment is typically based on these findings. Culture of sputum is used by some for the evaluation of pneumonia, Sputum collection is expectorated purulent matter from the lower respiratory tract and avoiding mouth and oropharyngeal matter for reducing contamination. Prior to culture, the specimen should be examined for the presence of WBCs (evidence of purulent matter) and a paucity of squamous cells (evidence of minimal contamination by oral matter).
 
Reference Value:
No growth or normal flora
 
Interpretation:
Isolate and identify potentially aerobic pathogenic organisms. Some bacterial agents that cause lower respiratory infections (eg, Mycobacteria, Legionella species, Mycoplasma pneumoniae) are not detected by this assay
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)