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Clear
 
Test Code (รหัสการทดสอบ):
090-40-0450-01

Order Name (ชื่อการทดสอบ):
Sputum C/S

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Sputum/ Sterile container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of bacterial infections
 
Methodology (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
AliasesName (ชื่อเรียกอื่นๆ) :
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
Sputum culture
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0450-01

Order Name (ชื่อการทดสอบ):
Sputum C/S

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Medications: Do not use antibiotics before collection of specimen
  1. Have patient rinse or gargle with water to remove excess oral biota. 
  2. Instruct patient to cough deeply to produce a lower respiratory specimen (not postnasal fluid)
  3. Collect in a sterile container
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Sputum/ Sterile container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Sputum, volume Entire collection (minimum 0.5 mL)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Sputum Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0450-01

Order Name (ชื่อการทดสอบ):
Sputum C/S

 
Method detail (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0450-01

Order Name (ชื่อการทดสอบ):
Sputum C/S

 
 
Clinical Information (ข้อมูลทางคลินิก):
Common bacterial organisms of acute pneumonia include: Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Pseudomonas aeruginosa, and members of the Enterobacteriaceae. Clinical history, physical examination, and chest X-ray are usually adequate for the diagnosis of pneumonia, and antimicrobial treatment is typically based on these findings. Culture of sputum is used by some for the evaluation of pneumonia, Sputum collection is expectorated purulent matter from the lower respiratory tract and avoiding mouth and oropharyngeal matter for reducing contamination. Prior to culture, the specimen should be examined for the presence of WBCs (evidence of purulent matter) and a paucity of squamous cells (evidence of minimal contamination by oral matter).
 
Reference Value (ค่าอ้างอิง):
No growth or normal flora
 
Interpretation (การแปลผล):
Isolate and identify potentially aerobic pathogenic organisms. Some bacterial agents that cause lower respiratory infections (eg, Mycobacteria, Legionella species, Mycoplasma pneumoniae) are not detected by this assay
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)