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Clear
 
Test Code:
090-71-5530

Order Name:
SARS-CoV-2 genotyping **

 
Useful For:
Clade identification of SARS-CoV-2 from positive-diagnostic specimen. THIS IS NOT A DIAGNOSTICS TEST FOR SARS-CoV-2. 
 
Methodology:
MassARRAY (Genotyping)
 
AliasesName:
COVID-19 genotyping
COVID
 
 
 
Test Code:
090-71-5530

Order Name:
SARS-CoV-2 genotyping **

 
Patient Preparation:
N/A
 
Collection Specimen Or Container:
Purified Viral RNA (RNA extraction) / Sterile vial - กรุณาแนบผล PCR
 
Specimen Testing Type:
Purified Viral RNA, minimum volume 20 uL
 
Sub Mission Container:
Sterile vial
 
 
 
Test Code:
090-71-5530

Order Name:
SARS-CoV-2 genotyping **

 
Method detail:
The cDNAs of specimen’s RNA were generated using the SuperScript™ IV First-Strand Synthesis System (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions. Subsequently, the amplification of PCR multiplex reactions was performed in the steps according to the manufacturer's protocol (Agena Bioscience, San Diego, CA, USA) using a proprietary set of primers in 36 positions designed for SARS-CoV-2 clade classification. The PCR products were then prepared as a matrix for the MassARRAY system (Agena Bioscience). Mass signals for the different alleles were captured with high accuracy by MALDI-TOF mass spectrometry. MassARRAY Typer v4.0 (Agena Bioscience, San Diego, CA, USA) was used to generate the genotyping data of the specimens.
 
Schedule:
N/A **Sent Out to Ramathibodi
 
Turnaround Time:
Received specimen to reported within 3 days
(exception for official holidays)
 
Performing Location:
Ramathibodi
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code:
090-71-5530

Order Name:
SARS-CoV-2 genotyping **

 
 
Reference Value:
Call rate < 50% indicates that a highly fragmented viral genome is suspected.
 
Interpretation:
Limitation: This test is designed for clade identification of SARS-CoV-2 from positive-diagnostic specimen. THIS IS NOT A DIAGNOSTICS TEST FOR SARS-CoV-2. Failure to classify could be from multiple reasons such as: a fragmented viral genome, or RNA degradation from inappropriate handling of the specimens, etc.
 
Clinical Reference:
Report of Center for Medical Genomics (Version: SARS_COV2_GEN_MAS_003)