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Clear
 
Test Code (รหัสการทดสอบ):
090-71-5530

Order Name (ชื่อการทดสอบ):
SARS-CoV-2 genotyping **

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Purified Viral RNA (RNA extraction) / Sterile vial - กรุณาแนบผล PCR
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days
(exception for official holiday)
 
Useful For (ประโยชน์การทดสอบ):
Clade identification of SARS-CoV-2 from positive-diagnostic specimen. THIS IS NOT A DIAGNOSTICS TEST FOR SARS-CoV-2. 
 
Methodology (วิธีการทดสอบ):
MassARRAY (Genotyping)
 
AliasesName (ชื่อเรียกอื่นๆ) :
COVID-19 genotyping
COVID
 
 
 
Test Code (รหัสการทดสอบ):
090-71-5530

Order Name (ชื่อการทดสอบ):
SARS-CoV-2 genotyping **

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
N/A
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Purified Viral RNA (RNA extraction) / Sterile vial - กรุณาแนบผล PCR
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Purified Viral RNA, minimum volume 20 uL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile vial
 
 
 
Test Code (รหัสการทดสอบ):
090-71-5530

Order Name (ชื่อการทดสอบ):
SARS-CoV-2 genotyping **

 
Method detail (วิธีการทดสอบ):
The cDNAs of specimen’s RNA were generated using the SuperScript™ IV First-Strand Synthesis System (Invitrogen, Carlsbad, USA) according to the manufacturer's instructions. Subsequently, the amplification of PCR multiplex reactions was performed in the steps according to the manufacturer's protocol (Agena Bioscience, San Diego, CA, USA) using a proprietary set of primers in 36 positions designed for SARS-CoV-2 clade classification. The PCR products were then prepared as a matrix for the MassARRAY system (Agena Bioscience). Mass signals for the different alleles were captured with high accuracy by MALDI-TOF mass spectrometry. MassARRAY Typer v4.0 (Agena Bioscience, San Diego, CA, USA) was used to generate the genotyping data of the specimens.
 
Schedule (ตารางการทดสอบ):
N/A **Sent Out to Ramathibodi
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days
(exception for official holiday)
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Ramathibodi
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code (รหัสการทดสอบ):
090-71-5530

Order Name (ชื่อการทดสอบ):
SARS-CoV-2 genotyping **

 
 
Reference Value (ค่าอ้างอิง):
Call rate < 50% indicates that a highly fragmented viral genome is suspected.
 
Interpretation (การแปลผล):
Limitation: This test is designed for clade identification of SARS-CoV-2 from positive-diagnostic specimen. THIS IS NOT A DIAGNOSTICS TEST FOR SARS-CoV-2. Failure to classify could be from multiple reasons such as: a fragmented viral genome, or RNA degradation from inappropriate handling of the specimens, etc.
 
Clinical Reference (เอกสารอ้างอิง):
Report of Center for Medical Genomics (Version: SARS_COV2_GEN_MAS_003)