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Test Code:
094-21-0014
094-21-0014-01

Order Name:
PD-L1 (Clone SP142), IHC, Tissue (FFPE) (Outside lab)

 
Useful For:
Identification of neoplasms expressing programmed cell death 1-ligand 1 (clone SP142)
 
Methodology:
Immunohistochemistry (IHC)
 
AliasesName:

Programmed Death-Ligand 1 (PD-L1) (SP142), Semi-Quantitative Immunohistochemistry, Manual
PD-L1 (SP142) SemiQuant IHC, Manual
PDL1
SP142
ISPDL1SP142IHC
GPPDL1SP142

 
 
 
Test Code:
094-21-0014
094-21-0014-01

Order Name:
PD-L1 (Clone SP142), IHC, Tissue (FFPE) (Outside lab)

 
Patient Preparation:
Necessary Information
A pathology/diagnostic report and a brief history, including primary site of neoplasm, are required.
 
Collection Specimen Or Container:
Tissue/ Formalin-fixed paraffin-embedded (FFPE) block
 
Specimen Testing Type:
Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, "positively charged" slides with 4-microns formalin-fixed, paraffin-embedded tissue.
Additional Information: One slide will be stained with hematoxylin and eosin.
 
Sub Mission Container:
1. Tissue in 10% neutral buffered formalin or
2. Paraffin embedded tissue block or
3. Unstained positively charged slides as least 3 slides with 4-microns formalin-fixed, paraffin-embedded tissue.
 
Rejection Criteria:
Wet/frozen tissue
Cytology smears
Nonformalin fixed tissue
Nonparaffin embedded tissue
Noncharged slides
ProbeOn slides
 
 
 
Test Code:
094-21-0014
094-21-0014-01

Order Name:
PD-L1 (Clone SP142), IHC, Tissue (FFPE) (Outside lab)

 
Method detail:
Immunohistochemistry (IHC)
 
Schedule:
N/A **Sent out to MAYO, USA
 
Turnaround Time:
Received specimen to report within 14 days
 
Performing Location:
MAYO Laboratory
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code:
094-21-0014
094-21-0014-01

Order Name:
PD-L1 (Clone SP142), IHC, Tissue (FFPE) (Outside lab)

 
 
Clinical Information:
Programmed cell death 1-ligand 1 (PD-L1), also known as B7 homolog 1 (B7-H1) or CD274, is a transmembrane protein involved in the regulation of cell-mediated immune responses through interaction with the receptor programmed death protein-1 (PD-1). PD-L1 has been identified as both a prognostic and theranostic marker in a variety of neoplasms. Overexpression of PD-L1 has been observed in carcinomas of the urinary bladder, lung, thymus, colon, pancreas, ovary, breast, kidney, and in melanoma and glioblastoma.
 
Interpretation:
The results of the test will be reported in form of scores. The scoring system is based on type and origin of tumor. 

Cautions:
Preclinical studies suggest that positive programmed cell death 1-ligand 1 (PD-L1) immunohistochemistry in tumor cells may predict tumor response to therapy with immune checkpoint inhibitors. This result should not be used as the sole factor in determining treatment, as other factors (eg, tumor mutation burden and microsatellite instability) have also been studied as predictive markers.

This test has been validated for nondecalcified paraffin-embedded tissue specimens fixed in 10% neutral-buffered formalin. This assay has not been validated on tissues subjected to the decalcification process or the use of alternative fixatives for bone and bone marrow specimens or cell blocks.

Age of a cut paraffin section can affect immunoreactivity. Stability thresholds vary widely among published literature and are antigen-dependent. Best practice is for paraffin sections to be cut within 6 weeks.
 
Clinical Reference:
www.mayomedicallaboratories.com (Retrieved: 4 Feb 2021)