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Test Code:
090-71-0501-01

Order Name:
PCR for Lyme Disease ( Blood ) **

 
Useful For:
Supporting the diagnosis of Lyme disease in conjunction with serologic testing.
 
 
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
 
Test List In Profile:
B. burgdorferi PCR
B. mayonii PCR
B. garinii/B. afzelii PCR
 
AliasesName:

Lyme Disease, Molecular Detection, PCR, Blood

 
 
 
Test Code:
090-71-0501-01

Order Name:
PCR for Lyme Disease ( Blood ) **

 
Patient Preparation:
N/A
 
Collection Specimen Or Container:
Blood/ K3 EDTA (Lavender Top) 3 mL, 2 tubes
เจาะเลือด วันอังคารหรือวันพุธก่อนเที่ยง เท่านั้น และ งดเจาะเลือดในวันหยุดนักขัตฤกษ์
 
 
Specimen Testing Type:
Whole blood EDTA, minimum volume 3 mL
 
 
Sub Mission Container:
K3 EDTA (Lavender Top)
 
Rejection Criteria:
Over stability when reach performing Lab at USA, Severe clotted specimen, Use heparin as anticoagulant
 
Specimen Stabillity:
Specimen Type Temperature Time
Whole blood, EDTA Refrigerated, 2oC to 8oC (preferred) 7 days **
Whole blood, EDTA Frozen  7 days **

** Sending to USA
 
 
 
Test Code:
090-71-0501-01

Order Name:
PCR for Lyme Disease ( Blood ) **

 
Method detail:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
 
Schedule:
N/A **Sent out to MAYO, USA
 
Turnaround Time:
Received specimen to reported within 14 days

 
 
Performing Location:
MAYO Laboratory
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code:
090-71-0501-01

Order Name:
PCR for Lyme Disease ( Blood ) **

 
 
Clinical Information:
Lyme disease is a multisystem and multistage tick-transmitted infection caused by spirochetal bacteria in the Borrelia burgdorferi sensu lato (Bbsl) complex.(1) Nearly all human infections are caused by 3 Bbsl species; B burgdorferi sensu stricto (hereafter referred to as B burgdorferi) is the primary cause of Lyme disease in North America, while B afzelii and B garinii are the primary causes of Lyme disease in Europe. In 2012, B mayonii was identified as a less common cause of Lyme disease in the upper Midwestern United States.(2,3) This organism has only been detected in patients with exposure to ticks in Minnesota and Wisconsin and has not been detected in over 10,000 specimens from patients in other states including regions of the northeast where Lyme disease is endemic.
 
Reference Value:
Negative
 
Interpretation:

A positive result indicates the presence of DNA from Borrelia burgdorferiB mayoniiB afzelii, or B garinii, the main agents of Lyme disease.

A negative result indicates the absence of detectable target DNA in the specimen. Due to the diagnostic sensitivity limitations of the PCR assay, a negative result does not preclude the presence of the organism or active Lyme disease.

 
Clinical Reference:
www.mayocliniclabs.com (Retrieved: 7 Jul 2020)