Oncomine Myeloid DNA-Blood

Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):

EDTA Blood 3ml, 1 tube, Lavender Top

For BH: Consent for Genomic Medicine Testing
For B2B: Molecular oncology test request form, Please click LINK

Turnaround Time (ระยะเวลารอผล):

Report within 14 days

Useful For (ประโยชน์การทดสอบ):

Oncomine Myeloid DNA assay is a next-generation sequencing (NGS)-based assay designed for comprehensive molecular profiling of myeloid malignancies, enabling the detection of clinically relevant genetic alterations associated with disease diagnosis, classification, prognosis, and therapeutic decision-making.

The assay detects single nucleotide variants (SNVs) and small insertions/deletions (InDels) across 52 genes implicated in a broad spectrum of myeloid neoplasms from blood sample, including:

Acute Myeloid Leukemia (AML)
Myelodysplastic Syndromes (MDS)
Myeloproliferative Neoplasms (MPN)
Chronic Myelomonocytic Leukemia (CMML)
Other hematologic malignancies with myeloid involvement

The identification of clinically significant and actionable genomic alterations can assist in:

Supporting the molecular diagnosis of myeloid malignancies
Disease classification and risk stratification according to current clinical guidelines
Prognostic assessment based on established molecular biomarkers
Selection of targeted therapies and personalized treatment strategies
Monitoring disease progression and informing long-term disease management

Gene List
DNA Hotspot Genes (32)

ABL1, ANKRD26, BRAF, CBL, CSF3R, DNMT3A, ETNK1, FLT3, GATA1, GATA2, HRAS, IDH1, IDH2, JAK2, KIT, KRAS, MPL, MYD88, NPM1, NRAS, PPM1D, PTPN11, SETBP1, SF3B1, SMC1A, SMC3, SRSF2, STAT3, STAT5B, U2AF1, UBA1, WT1

Full Gene Coverage (20)

ASXL1, BCOR, BCORL1, CALR, CEBPA, DDX41, ETV6, EZH2, IKZF1, NF1, PHF6, PRPF8, RAD21, RB1, RUNX1, SH2B3, STAG2, TET2, TP53, ZRSR2

Methodology (วิธีการทดสอบ):

Next generation sequencing (NGS),
Target sequencing

AliasesName (ชื่อเรียกอื่นๆ) :

Myeloid panel by Next Generation Sequencing (NGS)

Test Code (รหัสการทดสอบ):

092-10-0117

Order Name (ชื่อการทดสอบ):

Oncomine Myeloid DNA-Blood

Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):

EDTA Blood 3ml, 1 tube, Lavender Top

For BH: Consent for Genomic Medicine Testing
For B2B: Molecular oncology test request form, Please click LINK

Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):

Whole blood

Sub Mission Container (ภาชนะส่งตรวจ):

EDTA tube

Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):

EDTA Whole Blood, Refrigerated 2°C to 8°C for 3 days

Test Code (รหัสการทดสอบ):

092-10-0117

Order Name (ชื่อการทดสอบ):

Oncomine Myeloid DNA-Blood

Turnaround Time (ระยะเวลารอผล):

Report within 14 days

Performing Location (หน่วยงานที่ทำการทดสอบ):

Laboratory – Clinical Genomics Tel 14252, 14596

Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):

Store at -80°C within 3 months

Test Code (รหัสการทดสอบ):

092-10-0117

Order Name (ชื่อการทดสอบ):

Oncomine Myeloid DNA-Blood

Clinical Information (ข้อมูลทางคลินิก):

The Oncomine™ Myeloid DNA Assay GX v2 enables simultaneous detection of clinically relevant genetic alterations, including single nucleotide variants (SNVs) and insertions/deletions (InDels), across 52 genes associated with myeloid malignancies. The panel covers hotspot mutations and full-gene. The biomarkers included in the assay have been identified as frequently altered in a wide range of hematologic malignancies, including Acute Myeloid Leukemia (AML), Myelodysplastic Syndromes (MDS), Myeloproliferative Neoplasms (MPN), Chronic Myelomonocytic Leukemia (CMML), Juvenile Myelomonocytic Leukemia (JMML), and related myeloid disorders. Detection of these biomarkers may provide valuable information for disease classification, risk stratification, prognostic assessment, and therapeutic decision-making. Results should be interpreted in conjunction with clinical findings, laboratory data, cytogenetic studies, and other relevant diagnostic investigations.

Clinical Reference (เอกสารอ้างอิง):

1.Krishnamurthy K, Chai J, Liu X, Wang Y, Naeem R, Goldstein DY. Clinical validation of the Ion Torrent Oncomine Myeloid Assay GX v2 on the Genexus Integrated Sequencer as a stand-alone assay for single-nucleotide variants, insertions/deletions, and fusion genes: Challenges, performance, and perspectives. Am J Clin Pathol. 2024 Nov 4;162(5):480-491. doi: 10.1093/ajcp/aqae063. PMID: 38823030.

2.Zbieranski N, Insuasti-Beltran G. Analytical Validation of an Automated Semiconductor-Based Next-Generation Sequencing Assay for Detection of DNA and RNA Alterations in Myeloid Neoplasms. J Mol Diagn. 2024 Jan;26(1):29-36. doi: 10.1016/j.jmoldx.2023.09.011. Epub 2023 Oct 23. PMID: 37879438.