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Clear
 
Test Code (รหัสการทดสอบ):
090-10-0120-01

Order Name (ชื่อการทดสอบ):
Microfilaria

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
The specimens can be selected as list below:
  1. Blood/ K3 EDTA (K3E) (Lavender Top) 3 mL, 1 tube or
  2. Blood/ 2% Formalin Container, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours
 
Useful For (ประโยชน์การทดสอบ):
Detection of microfilariae in peripheral blood
 
Methodology (วิธีการทดสอบ):
Staining
 
AliasesName (ชื่อเรียกอื่นๆ) :
Elephantiasis
 
 
 
Test Code (รหัสการทดสอบ):
090-10-0120-01

Order Name (ชื่อการทดสอบ):
Microfilaria

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Certain of the microfilariae have a nocturnal periodicity, and the blood specimen is best taken at night between 10 p.m. and 2 a.m., Continuous 3 days
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
The specimens can be selected as list below:
  1. Blood/ K3 EDTA (K3E) (Lavender Top) 3 mL, 1 tube or
  2. Blood/ 2% Formalin Container, 1 tube
 
 
 
Test Code (รหัสการทดสอบ):
090-10-0120-01

Order Name (ชื่อการทดสอบ):
Microfilaria

 
Method detail (วิธีการทดสอบ):
Staining
 
Schedule (ตารางการทดสอบ):
Tested Daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
5 days
 
 
 
Test Code (รหัสการทดสอบ):
090-10-0120-01

Order Name (ชื่อการทดสอบ):
Microfilaria

 
 
Clinical Information (ข้อมูลทางคลินิก):
The filariae are parasitic nematodes (roundworms) that cause significant human morbidity in tropical regions worldwide. The macroscopic adults live in the human host and release microscopic offspring (microfilariae) into the blood or skin. The microfilariae of Wuchereria bancrofti, Brugia malayi, B timori, Loa loa, Mansonella perstans, and M ozzardi are found in the blood, while the microfilariae of Onchocerca volvulus and M streptocerca are found in the skin. If microfilariae are taken up by a biting insect vector (mosquitos, blackflies, midges, and deer flies), they undergo further development in the insect and can then be transmitted to other humans.

The microfilariae of these filarial worms can be seen on conventional thick and thin blood films, which allows for their definitive identification. However, microfilariae may be in low numbers and, therefore, use of concentration methods such as the Knott's technique improves the detection sensitivity. Some microfilariae are released into the blood at certain times of the day; W bancrofti and Brugia species are usually released between 10 p.m. and 2 a.m. (nocturnal periodicity), while L loa is released mostly from 10 a.m. and 2 p.m. (diurnal periodicity). It is therefore important to collect blood during these time periods for optimal detection sensitivity. Mansonella species microfilariae do not exhibit any periodicity and, therefore, a random blood draw is acceptable. Since the levels of parasitemia may fluctuate, multiple smears may be needed to detect the filarial worms. Blood should be obtained and examined every 8 to 12 hours for 2 to 3 days before excluding infection.
 
Reference Value (ค่าอ้างอิง):
Not found
 
Clinical Reference (เอกสารอ้างอิง):
http://www.mayomedicallaboratories.com (Retrieved: 22 Jan 2019)