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Clear
 
Test Code:
092-10-0005

Order Name:
KRAS reflect NRAS/BRAF mutation-FFPE (Idylla)

 
Useful For:
Detection of 21 relevant KRAS mutations in codons 12 and 13 (exon 2), codons 59 and 61 (exon 3) and codons 117 and 146 (exon 4) are performed. If KRAS mutation is not detected, further detection of 23 relevant mutations of NRAS and BRAF are performed that consist of 8 NRAS mutations in codons 12 and 13 (exon 2), 6 NRAS mutations in codons 59 and 61 (exon 3), 4 NRAS mutations in codons 117 and 146 (exon 4), 5 BRAF mutations in codon 600 (exon 15) directly from formalin-fixed paraffin-embedded (FFPE) tissue sections.
 
Methodology:
Real-time PCR (PlexZyme™ and PlexPrime™ technology, Idylla™ System)
 
Test List In Profile:
KRAS mutation-FFPE (Idylla)
NRAS/BRAF mutation-FFPE (Idylla)
 
AliasesName:
KRAS mutation
NRAS/BRAF mutation
RAS/RAF
All RAS
 
 
 
Test Code:
092-10-0005

Order Name:
KRAS reflect NRAS/BRAF mutation-FFPE (Idylla)

 
Collection Specimen Or Container:
Tissue/ Formalin-fixed paraffin-embedded (FFPE) block
 
Specimen Testing Type:
Tissue, Formalin-fixed paraffin-embedded (FFPE) block,
3 x 10 µm FFPE tissue section, 25 – 300 mm² (≥ 10% Neoplastic cell content)
 
Sub Mission Container:
Tissue/Formalin-fixed paraffin-embedded (FFPE) block
 
Rejection Criteria:
Neoplastic cell content <10%
 
Specimen Stabillity:
Specimen Type Temperature Time
FFPE Tissue section Ambient temperature 1 month
 
 
 
Test Code:
092-10-0005

Order Name:
KRAS reflect NRAS/BRAF mutation-FFPE (Idylla)

 
Method detail:
Real-time PCR (PlexZyme™ and PlexPrime™ technology, Idylla™ System)
 
Turnaround Time:
Received to Reported within 3 days
 
Performing Location:
Research and Development Tel. 14252
 
Specimen Retention Time:
1 month
 
 
 
Test Code:
092-10-0005

Order Name:
KRAS reflect NRAS/BRAF mutation-FFPE (Idylla)

 
 
Clinical Information:
"See details from individual unit test"
 
Clinical Reference:
"See details from individual unit test"