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Test Code:
090-70-1405-02

Order Name:
Influenza A/Influenza B/ RSV (RT-PCR) (Source specify)

 
Useful For:
Use for the qualitative detection and differentiation of influenza A, influenza B, and respiratory syncytial virus (RSV) viral RNA from patients with signs and symptoms of respiratory infection.
 
Methodology:
Multiplex real-time, reverse transcriptase polymerase chain reaction (RT-PCR) assay
 
Test List In Profile:
Influenza A Virus
nfluenza B Virus
Respiratory Syncytial Virus (RSV)
 
AliasesName:
PCR for Influenza A virus, Influenza B virus, RSV
Influenza A/B and RSV, PCR
Flu PCR
Respiratory Syncytial Virus (RSV), PCR
 
 
 
Test Code:
090-70-1405-02

Order Name:
Influenza A/Influenza B/ RSV (RT-PCR) (Source specify)

 
Collection Specimen Or Container:
  1. Throat swab/Universal Transport Media (UTM) 3 mL, 1 tube or
  2. Bronchoalveolar lavage (BAL)/Sterile Container or
  3. Bronchial washing/Sterile Container or
  4. Sputum/Sterile Container
 
Specimen Testing Type:
  1. Throat swab/ Universal Transport Media (UTM) 3 mL, 1 tube
  2. Bronchoalveolar lavage (BAL), 5-10 mL (Minimum volume 1.5 mL)
  3. Bronchial washing, 5-10 mL (Minimum volume 1.5 mL)
  4. Sputum, 2-3 mL (Minimum volume 0.5 mL)
 
Sub Mission Container:
Universal Transport Media (UTM) tube or Sterile Container
 
Rejection Criteria:
  1. Specimen is added any preservative solution
  2. Sputum is mostly saliva not true sputum.
 
Specimen Stabillity:
Specimen Type Temperature Time
Throat swab Refrigerated, 2oC to 8oC 7 days
Bronchoalveolar lavage (BAL)
Bronchial washing
Sputum
 
 
 
Test Code:
090-70-1405-02

Order Name:
Influenza A/Influenza B/ RSV (RT-PCR) (Source specify)

 
Method detail:
Multiplex real-time, reverse transcriptase polymerase chain reaction (RT-PCR) assay
 
Schedule:
24 hours service
 
Turnaround Time:
Received specimen to reported within 1 hr.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-70-1405-02

Order Name:
Influenza A/Influenza B/ RSV (RT-PCR) (Source specify)

 
 
Clinical Information:
Influenza, or the flu, is a contagious viral infection of the respiratory tract. Transmission of influenza is primarily airborne (i.e., coughing or sneezing); the peak of transmission usually occurs in the winter months. Symptoms commonly include fever, chills, headache, muscle aches, malaise, cough, and sinus congestion. Gastrointestinal symptoms (i.e., nausea, vomiting, or diarrhea) may also occur, primarily in children, but are less common in adults. Symptoms generally appear within two days of exposure to an infected person. Pneumonia may develop as a complication of influenza infection, causing increased morbidity and mortality in pediatric, elderly, and immunocompromised populations.
Respiratory syncytial virus (RSV) is the cause of a contagious disease that afflicts primarily infants and the elderly who are immunocompromised. The virus can live for hours on countertops and toys and causes both upper respiratory infections, such as tracheobronchitis and lower respiratory infections manifesting as bronchiolitis and pneumonia.
 
Reference Value:
Test Reference Value
Influenza A Virus Negative
Influenza B Virus Negative
Respiratory Syncytial Virus (RSV) Negative
 
Interpretation:
  1. This assay result should be interpreted with other laboratory and clinical data available to the clinician evaluating the patient.
  2. Erroneous test results might occur from improper specimen collection, failure to follow the recommended sample collection, handling and storage procedures, technical error, sample mix-up, or because the number of organisms in the specimen is too low to be detected by the test.
  3. Negative results do not preclude influenza virus or RSV infection and should not be used as the sole basis for treatment or other patient management decisions.
  4. Viral nucleic acid may persist in vivo, independent of virus viability. Detection of analyte target(s) does not imply that the corresponding virus(es) are infectious or are the causative agents for clinical symptoms.
  5. If the virus mutates in the target region, influenza virus and RSV may not be detected or may be detected less predictably.
  6. The test is specific for influenza A, influenza B, and RSV; therefore, the results do not exclude the possibility of infection with other respiratory viruses.
 
Clinical Reference:
Manufacturer’s packages insert, Xpert® Xpress Flu/RSV, April 2019, Cepheid, CA 94089 Sunnyvale, USA.