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Test Code (รหัสการทดสอบ):
090-71-0006

Order Name (ชื่อการทดสอบ):
HIV-1 Drug Resistant Genotype (Protease & RT & IN) **

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 2 tubes
 
Turnaround Time (ระยะเวลารอผล):
14 days
 
Useful For (ประโยชน์การทดสอบ):
To manage HIV-1 treatment.
This test is intended to be used to monitor known HIV-positive infections. It is not intended for primary detection of HIV infections.
Specimens should contain a minimum HIV viral load of 1000 copies/ml. Testing may be cancelled if the specimen supplied is inadequate.
 
Methodology (วิธีการทดสอบ):
Amplify HIV-1 protease, reverse transcriptase and integrase region, then genotyping by direct sequencing method. Compare the sequence with Stanford database.
 
Test List In Profile (การทดสอบใน Profile):
PI (Protease Inhibitor)
RTI (Reverse transcriptase inhibitor)
IN (Integrase inhibitor)
 
AliasesName (ชื่อเรียกอื่นๆ) :
HIV-1 drug resistance (PI+RTI+INI)
Human Immunodeficiency Virus Genotype
 
 
 
Test Code (รหัสการทดสอบ):
090-71-0006

Order Name (ชื่อการทดสอบ):
HIV-1 Drug Resistant Genotype (Protease & RT & IN) **

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 2 tubes
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Plasma EDTA, minimum volume 2 mL - Separate plasma within 6 hours of collection.
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Plasma, EDTA Refrigerated, 2oC to 8oC 5 days
Frozen 35 days
 
 
 
Test Code (รหัสการทดสอบ):
090-71-0006

Order Name (ชื่อการทดสอบ):
HIV-1 Drug Resistant Genotype (Protease & RT & IN) **

 
Method detail (วิธีการทดสอบ):
Amplify HIV-1 protease, reverse transcriptase and integrase region, then genotyping by direct sequencing method. Compare the sequence with Stanford database.
 
Schedule (ตารางการทดสอบ):
N/A **Sent Out to N Health
 
Turnaround Time (ระยะเวลารอผล):
14 days
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
N Health
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code (รหัสการทดสอบ):
090-71-0006

Order Name (ชื่อการทดสอบ):
HIV-1 Drug Resistant Genotype (Protease & RT & IN) **

 
 
Clinical Information (ข้อมูลทางคลินิก):
HIV-1 is an RNA virus that infects cells and is then converted to complementary DNA (cDNA) by the action of the viral reverse transcriptase (RT). RT has little proofreading capacity and therefore incorporates errors in the proviral DNA. These errors are transcribed into infectious viral particles when the proviral DNA is transcribed into RNA. Similarly, the enzyme protease (PR) catalyzes a polyprotein to produce peptides necessary for active viral replication. Although HAART (combinations of nucleoside analogs, nonnucleoside agents, protease inhibitors and/or integrase strand transfer inhibitors) may be effective in reducing viral load, genotypic mutations arising in drug-targeted HIV loci due to selective pressure from antiviral therapy can result in antiviral resistance that may compromise such therapy.
 
Reference Value (ค่าอ้างอิง):
N/A
 
Interpretation (การแปลผล):
Detectable HIV-1 genotypic mutations conferring resistance to an antiviral drug are reported as amino acid codon changes (eg, N155H), along with associated resistance interpretations for the current FDA-approved integrase strand transfer inhibitors (dolutegravir, elvitegravir, and raltegravir). Interpretation for resistance to bictegravir is not available at present.
 
Clinical Reference (เอกสารอ้างอิง):
  1. N Health Laboratory Catalog 2017
  2. www.mayomedicallaboratories.com (Retrieved: 24 Jan 2019)