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Test Code:
F8

Order Name:
Factor VIII activity assay

 
Useful For:
Evaluate spontaneous or prolonged bleeding suggestive of a coagulation disorder, Diagnosing hemophilia A
 
Methodology:
Clotting assay
 
AliasesName:
Coagulation Factor VIII Assay
Plasma Factor VIII Activity Assay
Hemophilia A
 
 
 
Test Code:
F8

Order Name:
Factor VIII activity assay

 
Collection Specimen Or Container:
Blood / Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 1 tube
 
Specimen Testing Type:
Citrate plasma, minimum volume 0.5 mL
 
Sub Mission Container:
Plastic tube
 
Rejection Criteria:
Hemolysis: Hemoglobin > 500 mg/dL reject
Lipemia: Triglyceride > 1000 mg/dL reject
Icterus: Bilirubin > 26 mg/dL
Other: Clotted specimen will be rejected.
 
Specimen Stabillity:
Specimen Type Temperature Time
Whole blood, Sodium citrate Room temperature, 18oC to 25oC 4 hours
Plasma, Sodium citrate Refrigerated, 2oC to 8oC 4 hours
Room temperature, 18oC to 25oC 4 hours
Frozen, -20oC 2 weeks
Frozen, -70oC 6 months
 
 
 
Test Code:
F8

Order Name:
Factor VIII activity assay

 
Method detail:
Clotting assay
 
Schedule:
Tested Daily (24 hours)
 
Turnaround Time:
Collected specimen to report within 3 hours
 
Performing Location:
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time:
14 days
 
 
 
Test Code:
F8

Order Name:
Factor VIII activity assay

 
 
Clinical Information:
The factor VIII gene is located on the X chromosome and deficiency is most commonly the result of inheritance of a faulty gene in a sex-linked recessive pattern. Factor VIII deficiency, however, may also be the result of spontaneous mutation, or from acquired deficiency due to the presence of autoantibodies directed against factor VIII. Regardless of cause, the bleeding disorder caused by deficiency of factor VIII is known as Hemophilia A
 
Reference Value:
50 - 150% Activity
 
Clinical Reference:
  1. Manufacturer’s reagent package insert, HemosIL™ Factor VIII deficient plasma - 0020011800, Insert revision 09/2016, Instrumentation Laboratory Company - Bedford, MA 01730-2443 (USA), Instrumentation Laboratory SpA - V.le Monza 338 - 20128 Milano (Italy)
  2. https://www.mayocliniclabs.com (Retrieved: 22 Jan 2019)