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Clear
 
Test Code:
090-40-0260-01

Order Name:
Eye C/S

 
Useful For:
Diagnosis of bacterial infections
 
Methodology:
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
AliasesName:
Aerobic culture
Aerobic bacteria culture
Culture, General Bacteria
Bacteria cullture
 
 
 
Test Code:
090-40-0260-01

Order Name:
Eye C/S

 
Patient Preparation:
Medications: Do not use antibiotics before collection of specimen
 
Collection Specimen Or Container:
Eye/ Media plate or Stuart transport medium or Sterile screw-cap tube

Conjunctiva
  1. Sample eye with  swab (premoistened with sterile saline) by rolling over each conjunctiva
  2. Swab directly onto media plate (Blood agar and Chocolate agar) or Stuart transport medium
  3. Smear for Gram stain may be prepared at time of collection. Roll swab over 1- to 2-cm area of slide
Vitreous fluid
  1. Prepare eye for needle aspiration of fluid
  2. inoculation into Sterile screw-cap tube or inoculation of fluid directly onto media plate

Corneal scrapings
  • See details from Corneal scrapings C/S
 
Specimen Testing Type:
Eye, volume Entire collection
 
Sub Mission Container:
Media plate or Stuart transport medium or Sterile screw-cap tube
 
Rejection Criteria:
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Eye
(Conjunctiva/ Vitreous fluid)
Direct media plate Room temperature, 18oC to 25oC 15 minutes
Stuart transport medium Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0260-01

Order Name:
Eye C/S

 
Method detail:
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
  3. Suceptibility test by Vitek 2 Compact
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 3 days.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0260-01

Order Name:
Eye C/S

 
 
Clinical Information:
Eye infections are usually caused by bacteria, fungi and viruses. Bacterial conjunctivitis is more common in children. The most common bacteria are Haemophilus influenza, Streptococcus pneumoniae and Staphylococcus aureus.
 
Reference Value:
No growth or normal flora
 
Interpretation:
Any microorganism found where normal flora is not present is considered significant and is reported. For specimens contaminated with the normal bacterial flora, bacteria that are potentially pathogenic are identified.
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)