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Test Code (รหัสการทดสอบ):
BILICNJ

Order Name (ชื่อการทดสอบ):
Direct Bilirubin

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to report within 1.5 hours (90 mins)
 
Useful For (ประโยชน์การทดสอบ):
Evaluation of jaundice and liver functions
 
Methodology (วิธีการทดสอบ):
Diazo Reaction
 
 
 
Test Code (รหัสการทดสอบ):
BILICNJ

Order Name (ชื่อการทดสอบ):
Direct Bilirubin

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Serum, minimum volume 0.5 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic vial
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: 1+ or above reject
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 7 days
Frozen, -20o 3 months

 
 
 
 
Test Code (รหัสการทดสอบ):
BILICNJ

Order Name (ชื่อการทดสอบ):
Direct Bilirubin

 
Method detail (วิธีการทดสอบ):
Diazo Reaction
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to report within 1.5 hours (90 mins)
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Chemistry, Laboratory Department Tel. 13224
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
5 days
 
 
 
Test Code (รหัสการทดสอบ):
BILICNJ

Order Name (ชื่อการทดสอบ):
Direct Bilirubin

 
 
Clinical Information (ข้อมูลทางคลินิก):
Red blood cells at the end of their circulating life are broken down in the reticuloendothelial system, mainly the spleen. The resulting heme, once the iron is removed, is then converted to bilirubin. This process accounts for about 80% of the 500 μmol (300 mg) of bilirubin formed daily. Other sources of bilirubin include the breakdown of myoglobin and cytochromes and the catabolism of immature red blood cells in the bone marrow. Once formed, bilirubin is transported to the liver bound to albumin. This fraction of bilirubin is referred to as indirect or unconjugated bilirubin. In the liver, bilirubin is conjugated to glucuronic acid (mono- and diglucuronides) to form conjugated bilirubin by the enzyme uridyl diphosphate glucuronyl transferase. Conjugated bilirubin or direct bilirubin is excreted via the biliary system into the intestine, where it is metabolized by bacteria to a group of products known collectively as stercobilinogen. Elimination is almost complete and serum levels are normally negligible. Direct bilirubin is the sum of the conjugated fractions. Direct bilirubin is elevated in conditions causing hepatic obstruction, hepatitis, cirrhosis, several inherited enzyme deficiencies, and inherited defects in canalicular excretion.
 
Reference Value (ค่าอ้างอิง):
0 – 0.5 mg/dL
 
Interpretation (การแปลผล):
Direct bilirubin levels must be assessed in conjunction with total and indirect levels and the clinical setting.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s reagent package insert, Architect® Direct Bilirubin, ABBOTT Laboratories, Abbott Park IL 60064 USA, February 2017.
  2. http://www.mayomedicallaboratories.com (Retrieved: 01 Jan 2019)