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Test Code:
BILICNJ

Order Name:
Direct Bilirubin

 
Useful For:
Evaluation of jaundice and liver functions
 
Methodology:
Diazo Reaction
 
 
 
Test Code:
BILICNJ

Order Name:
Direct Bilirubin

 
Collection Specimen Or Container:
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 0.5 mL
 
Sub Mission Container:
Plastic vial
 
Rejection Criteria:
Hemolysis: 1+ or above reject
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 7 days
Frozen, -20o 3 months

 
 
 
 
Test Code:
BILICNJ

Order Name:
Direct Bilirubin

 
Method detail:
Diazo Reaction
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Collected specimen to report within 1.5 hours (90 mins)
 
Performing Location:
Chemistry, Laboratory Department Tel. 13224
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
BILICNJ

Order Name:
Direct Bilirubin

 
 
Clinical Information:
Red blood cells at the end of their circulating life are broken down in the reticuloendothelial system, mainly the spleen. The resulting heme, once the iron is removed, is then converted to bilirubin. This process accounts for about 80% of the 500 μmol (300 mg) of bilirubin formed daily. Other sources of bilirubin include the breakdown of myoglobin and cytochromes and the catabolism of immature red blood cells in the bone marrow. Once formed, bilirubin is transported to the liver bound to albumin. This fraction of bilirubin is referred to as indirect or unconjugated bilirubin. In the liver, bilirubin is conjugated to glucuronic acid (mono- and diglucuronides) to form conjugated bilirubin by the enzyme uridyl diphosphate glucuronyl transferase. Conjugated bilirubin or direct bilirubin is excreted via the biliary system into the intestine, where it is metabolized by bacteria to a group of products known collectively as stercobilinogen. Elimination is almost complete and serum levels are normally negligible. Direct bilirubin is the sum of the conjugated fractions. Direct bilirubin is elevated in conditions causing hepatic obstruction, hepatitis, cirrhosis, several inherited enzyme deficiencies, and inherited defects in canalicular excretion.
 
Reference Value:
0 – 0.5 mg/dL
 
Interpretation:
Direct bilirubin levels must be assessed in conjunction with total and indirect levels and the clinical setting.
 
Clinical Reference:
  1. Manufacturer’s reagent package insert, Architect® Direct Bilirubin, ABBOTT Laboratories, Abbott Park IL 60064 USA, February 2017.
  2. http://www.mayomedicallaboratories.com (Retrieved: 01 Jan 2019)