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Test Code (รหัสการทดสอบ):
090-41-1501-01

Order Name (ชื่อการทดสอบ):
Cryptosporidium Antigen, Stool **

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Stool, Sterile container


 
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 14 days
 
 
Useful For (ประโยชน์การทดสอบ):

Establishing the diagnosis of intestinal cryptosporidiosis.

 
Methodology (วิธีการทดสอบ):
Enzyme-Linked Immunosorbent Assay (ELISA)
 
 
 
Test Code (รหัสการทดสอบ):
090-41-1501-01

Order Name (ชื่อการทดสอบ):
Cryptosporidium Antigen, Stool **

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
N/A
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Stool, Sterile container


 
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Stool, minimum volume 5 g (Ship Frozen)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):


 
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Stool  Frozen 60 days

 
 
 
 
Test Code (รหัสการทดสอบ):
090-41-1501-01

Order Name (ชื่อการทดสอบ):
Cryptosporidium Antigen, Stool **

 
Method detail (วิธีการทดสอบ):
Enzyme-Linked Immunosorbent Assay (ELISA)
 
Schedule (ตารางการทดสอบ):
N/A **Sent out to MAYO, USA
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 14 days
 
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
MAYO Laboratory
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code (รหัสการทดสอบ):
090-41-1501-01

Order Name (ชื่อการทดสอบ):
Cryptosporidium Antigen, Stool **

 
 
Clinical Information (ข้อมูลทางคลินิก):

Cryptosporidia are small apicomplexan protozoan parasites that infect the intestinal tract of humans and animals. They were conventionally categorized as coccidia, but are now known to be more closely related to the gregarines. Many species may infect humans, with the most common being Cryptosporidium hominis and C parvum. Infected humans and animals shed small (4-6 micrometer in diameter) infectious oocysts in their stool, and these can subsequently contaminate and survive in recreational and drinking water supplies.
Infection of humans occurs by the fecal-oral route or via ingestion of contaminated water or food. Infection is easily acquired, with an infectious dose of approximately 100 oocysts. Waterborne transmission is a primary mode of transmission and is commonly responsible for human outbreaks. This is due to the fact that Cryptosporidium species oocysts are resistant to cold temperatures and chlorine, and require extensive filtration or water treatment to remove them from drinking water.
The incubation period is typically 7 to 10 days following exposure. While most patients have symptoms, approximately 30% of infected individuals are asymptomatic. When symptoms are present, they usually include profuse watery diarrhea, malaise, anorexia, nausea, crampy abdominal pain, and low grade fever. Infection is usually self-limited in immunocompetent individuals, with resolution of symptoms in 10 to 14 days. However, diarrhea can be prolonged and life-threatening in immunocompromised patients such as those with AIDS, infants, and the elderly, and result in severe dehydration and wasting.
The fecal ova and parasite examination is an insensitive method for detecting Cryptosporidium, given the small size of the oocysts and their lack of trichrome staining. Instead, use of the Cryptosporidium antigen test (CRYPS / Cryptosporidium Antigen, Feces) or the multiplex gastrointestinal PCR panel (GIP / Gastrointestinal Pathogen Panel, PCR, Feces) is recommended for sensitive and specific detection. The antigen test is ideal for situations in which cryptosporidiosis is highly suspected (eg outbreak scenarios), whereas the PCR panel allows for simultaneous detection of multiple parasitic, viral, and bacterial causes of diarrhea.

 
Reference Value (ค่าอ้างอิง):
Negative
 
Interpretation (การแปลผล):

A positive enzyme-linked immunosorbent assay (ELISA) indicates the presence of antigens of cryptosporidium and is interpreted as evidence of infection with that organism.
The sensitivity, specificity, and positive predictive value of the ELISA were 87%, 99%, and 98%, respectively, as determined by examination of 231 fecal specimens by conventional microscopy and by ELISA.

 
Clinical Reference (เอกสารอ้างอิง):
www.mayomedicallaboratories.com (Retrieved: 25 Jun 2020)