bih.button.backtotop.text
BROWSE BY TEST NAME
%
1
2
3
5
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Clear
 
Test Code:
CORTR

Order Name:
Cortisol

 
Useful For:
  1. Determination of cortisol in human serum.
  2. Use as an aid in the diagnosis and treatment of adrenal disorders.
 
Methodology:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
 
 
Test Code:
CORTR

Order Name:
Cortisol

 
Collection Specimen Or Container:
Blood/ Plain Blood (Red Top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 1 mL
 
Sub Mission Container:
Plastic vial
 
Rejection Criteria:
Hemolysis: 4+ reject
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum (keep in original tube) Refrigerated, 2oC to 8oC 8 hours
Serum Refrigerated, 2oC to 8oC 14 days
Frozen, -20oC 1 month
 
 
 
Test Code:
CORTR

Order Name:
Cortisol

 
Method detail:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Specimen collected to reported within 2:00 hours (120 mins)
 
 
Performing Location:
Immunology, Laboratory Department Tel. 13227
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
CORTR

Order Name:
Cortisol

 
 
Clinical Information:
Cortisol is the major glucocorticoid hormone secreted by the adrenal cortex. Its physiological functions include regulation of carbohydrate metabolism and electrolyte and water distribution. Cortisol also has immunosuppressive and anti-inflammatory activity. In normal individuals, cortisol levels are regulated through a negative feedback loop in which the adrenal cortex responds to increased adrenocorticotropic hormone (ACTH) levels by increasing cortisol secretion, and the pituitary responds to elevated cortisol levels by down-regulation of ACTH production. Plasma cortisol levels are highest in the morning, and concentrations decrease by about half toward evening. Pregnancy or estrogen treatment markedly elevates cortisol levels. Other stimuli such as severe stress may also lead to increased cortisol production.

Cortisol measurements are used as a direct monitor of adrenal status and an indirect measure of pituitary hyper or hypofunction. Elevated cortisol levels are associated with adrenal tumors, pituitary tumors or
ectopic ACTH-producing tumors. Subnormal cortisol concentrations may indicate generalized adrenal hypofunction or a defect in the metabolic pathway for cortisol biosynthesis. The majority of cortisol in plasma is
bound to proteins and approximately 1% is excreted unchanged into the urine. Urinary cortisol is generally thought to reflect the level of unbound (free) plasma cortisol, which is biologically active. In cases of cortisol
overproduction, cortisol-binding globulin becomes saturated, such that unbound plasma cortisol increases disproportionately, as does urinary excretion. The measurement of urinary cortisol is a sensitive means of determining adrenocortical hyperfunction such as Cushing’s syndrome.

Urinary cortisol from 24-hour collections represent integration over a full day and are not affected by the diurnal variation evident in plasma cortisol levels. Cortisol measurements are often performed in conjunction with certain “challenge” tests designed to measure whether regulation of the hypothalamic-pituitary-adrenal axis is intact. These include the dexamethasone suppression test (DST), ACTH stimulation test and insulin tolerance test. Such challenge tests aid in the differential diagnosis of Cushing’s syndrome (cortisol overproduction) and the assessment of Addison’s disease (cortisol underproduction).
 
Reference Value:
Cortisol
Time Reference value Unit
Before 10 a.m. 3.7 - 19.4 µg/dL
After 5 p.m. 2.9 - 17.3 µg/dL
 
Clinical Reference:
Manufacturer’s Reagent package insert Architect  Cortisol, November 2015, Abbott Laboratories, Abbott Park, IL 60064, USA.