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Test Code (รหัสการทดสอบ):
090-10-1044-01

Order Name (ชื่อการทดสอบ):
CD Profile (CD3/CD4/CD8)

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood / K3 EDTA (K3E) (Lavender Top) 3 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to reported within 1 day.
 
Useful For (ประโยชน์การทดสอบ):
Useful for follow-up and diagnostic evaluation of primary cellular immunodeficiencies, including severe combined immunodeficiency.

T-cell immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized.

Assessment of T-cell immune reconstitution post hematopoietic cell transplantation.

Early screening of gross quantitative anomalies in T cells in infection or malignancies
 
Methodology (วิธีการทดสอบ):
Flow cytometry
 
Test List In Profile (การทดสอบใน Profile):
CD3 Cell Count
% CD3
CD4 Cell Count
% CD4
CD8 Cell Count
% CD8
CD4/CD8 Ratio
 
AliasesName (ชื่อเรียกอื่นๆ) :
Quantitative CD4 and CD8
T-Helper/T-Suppressor-Flow Cytometry
T4/T8 Helper Suppressor Ratio
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1044-01

Order Name (ชื่อการทดสอบ):
CD Profile (CD3/CD4/CD8)

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood / K3 EDTA (K3E) (Lavender Top) 3 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Blood, minimum volume 2 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Original tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Clotted specimen will be rejected.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Whole blood, EDTA Room temperature, 18oC to 24oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1044-01

Order Name (ชื่อการทดสอบ):
CD Profile (CD3/CD4/CD8)

 
Method detail (วิธีการทดสอบ):
Flow cytometry
 
Schedule (ตารางการทดสอบ):
Tested Daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Collected specimen to reported within 1 day.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Hematology, Laboratory Department Tel. 17254
 
 
 
Test Code (รหัสการทดสอบ):
090-10-1044-01

Order Name (ชื่อการทดสอบ):
CD Profile (CD3/CD4/CD8)

 
 
Clinical Information (ข้อมูลทางคลินิก):
Abnormalities in the number and percent of CD3, CD4, and CD8 T cells have been described in a number of different disease conditions. In patients who are infected with HIV, the CD4 count is measured for AIDS diagnosis and for initiation of antiviral therapy. The progressive loss of CD4 T lymphocytes in patients infected with HIV is associated with increased infections and complications. The Public Health Service has recommended that all HIV-positive patients be tested every 3 to 6 months for the level of CD4 T lymphocytes.

Basic T-cell subset quantitation is also very useful in the evaluation of patients with primary cellular immunodeficiencies of all ages, including follow-up for newborn screening for severe combined immunodeficiency and immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, or any other relevant clinical condition where immunomodulatory treatment is used, and the T-cell compartment is specifically affected.

It is also helpful as a preliminary screening assay for gross quantitative anomalies in T cells, whether related to malignancies or infection.
 
Reference Value (ค่าอ้างอิง):
Test Reference Value Unit
CD3 Cell Count 53.6 - 83.8 %
847 - 2347 cell.cu.mm
CD4 Cell Count 26.4 - 56.0 %
446 - 1327 cell.cu.mm
CD8 Cell Count 15.1 - 39.9 %
255 - 1016 cell.cu.mm
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s reagent package insert, CYTO-STAT® triChrome CD45-FITC/ CD4-RD1/CD3-PC5 REF 6607015. Beckman Coulter, Inc. U.S.A. 06/2016
  2. Manufacturer’s reagent package insert , CYTO-STAT® triChrome CD45-FITC/ CD8-RD1/CD3-PC5 REF 6607017. Beckman Coulter, Inc. U.S.A. 2014v
  3. http://www.mayomedicallaboratories.com (Retrieved: 22 Jan 2019)