Test Code (รหัสการทดสอบ):
090-70-1470
Order Name (ชื่อการทดสอบ):
Blood culture identification (BCID) by FilmArray, PCR
Clinical Information (ข้อมูลทางคลินิก):
Bloodstream infections (BSIs) occur when pathogenic organisms (e.g., bacteria or yeast) enter the bloodstream and cause disease. These infections are commonly identified by the growth of the pathogenic organism in blood culture. BSIs can lead to the development of sepsis, defined as life-threatening organ dysfunction caused by a dysregulated host response1 to infection.
Timely diagnosis and administration of effective treatment can significantly reduce mortality, duration of hospital stays, and costs due to BSI and sepsis.
Reference Value (ค่าอ้างอิง):
| Enterococcus faecalis |
Not Detected |
| Enterococcus faecium |
Not Detected |
| Listeria monocytogenes |
Not Detected |
| Staphylococcus spp. |
Not Detected |
| Staphylococcus aureus |
Not Detected |
| Staphylococcus epidermidis |
Not Detected |
| Staphylococcus lugdunensis |
Not Detected |
| Streptococcus spp. |
Not Detected |
| Streptococcus agalactiae (Group B) |
Not Detected |
| Streptococcus pneumoniae |
Not Detected |
| Streptococcus pyogenes (Group A) |
Not Detected |
| Acinetobacter calcoaceticus-baumannii complex |
Not Detected |
| Bacteroides fragilis |
Not Detected |
| Enterobacterales |
Not Detected |
| - Enterobacter cloacae complex |
Not Detected |
| - Escherichia coli |
Not Detected |
| - Klebsiella aerogenes |
Not Detected |
| - Klebsiella oxytoca |
Not Detected |
| - Klebsiella pneumoniae group |
Not Detected |
| - Proteus spp. |
Not Detected |
| - Salmonella spp. |
Not Detected |
| - Serratia marcescens |
Not Detected |
| Haemophilus influenzae |
Not Detected |
| Neisseria meningitidis |
Not Detected |
| Pseudomonas aeruginosa |
Not Detected |
| Stenotrophomonas maltophilia |
Not Detected |
| Candida albicans |
Not Detected |
| Candida auris |
Not Detected |
| Candida glabrata |
Not Detected |
| Candida krusei |
Not Detected |
| Candida parapsilosis |
Not Detected |
| Candida tropicalis |
Not Detected |
| Cryptococcus neoformans/gattii |
Not Detected |
Interpretation (การแปลผล):
1. All BioFire BCID2 Panel results are intended to be interpreted in conjunction with Gram stain results. In some cases, the Gram stain result and the BioFire BCID2 Panel result may be discrepant. In these cases, the BioFire BCID2 Panel results should be confirmed, e.g., by culture or other laboratory, epidemiological, or clinical findings. Blood culture media may contain non-viable organisms and/or nucleic acids that may lead to false positive BioFire BCID2 Panel results. Typically, these false positives present with more than one positive result from the BioFire BCID2 Panel.
2. Only encapsulated strains of N. meningitidis will be detected by the BIOFIRE BCID2 Panel. Unencapsulated N. meningitidis
will not be detected.
3. The BIOFIRE BCID2 Panel may not distinguish mixed cultures when two or more species of the same genus or organism group are present in a specimen (e.g., Staphylococcus aureus and Staphylococcus hominis).
4. In mixed cultures, the BIOFIRE BCID2 Panel may not identify all targeted organisms in the specimen, depending upon the concentration of each target present. In particular, false negative results for Pseudomonas aeruginosa or Stenotrophomonas spp. may occur if another organism is present in the blood culture. Conversely, standard subculture methods may also not identify all organisms in a mixed culture, depending upon the concentration and growth characteristics of each organism present.
5. Antimicrobial resistance can occur via multiple mechanisms. A Not Detected result for the antimicrobial resistance gene assays does not indicate antimicrobial susceptibility. Subculturing and standard susceptibility testing of isolates are required to determine antimicrobial susceptibility.
6. A negative BIOFIRE BCID2 Panel result does not exclude the possibility of bloodstream infection. Negative test results may occur from sequence variants in the region targeted by the assay, the presence of inhibitors, technical error, sample mix-up, or an infection caused by an organism not detected by the panel. Test results may also be affected by concurrent antibacterial/antifungal therapy or levels of organism in the sample that are below the limit of detection for the test (especially in the case of mixed cultures). Negative results should not be used as the sole basis for diagnosis, treatment, or other management decisions.
7. The vanA/B result is not reported in the absence of Enterococcus faecalis or Enterococcus faecium detection and will therefore not be reported for blood cultures containing other vancomycin-resistant Enterococci or vancomycin-resistant Staphylococcus aureus (VRSA).
8. False positives and false negatives can be the result of a variety of sources and causes. It is important that results be used in conjunction with other clinical, epidemiological, or laboratory information.
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s package inserts. FilmArray Blood Culture Identification 2 (BCID2) Panel, January 2025, BioFire Diagnostics, Salt Lake City, UT 84108, USA.