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Test Code:
AT3

Order Name:
Antithrombin III

 
Useful For:
Exclude or diagnose hereditary deficiency in patients with a tendency toward thromboembolism, in pre-operative stages, before prescription of oral contraceptives, DIC, nephrotic syndromes, liver diseases and in therapy with heparin or antithrombin concentrates.
 
Methodology:
Chromogenic substrate
 
AliasesName:
Antithrombin Activity
AT3 Activity/Functional
AT III Activity/Functional
Anti-Thrombin III
 
 
 
Test Code:
AT3

Order Name:
Antithrombin III

 
Collection Specimen Or Container:
Blood/ Sodium citrate tube (Sodium Citrate 3.2% anticoagulant, Blue top) 3 mL, 2 tubes
 
Specimen Testing Type:
Citrate plasma, minimum volume 0.5 mL
 
Sub Mission Container:
Plastic tube
 
Rejection Criteria:
Hemolysis: Hemoglobin > 500 mg/dL
Lipemia: Triglyceride > 2,300 mg/dL
Icterus: Bilirubin > 40 mg/dL
Other: Clotted specimen will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Whole blood Room temperature, 18oC to 25oC 4 hours
Platelet-poor plasma Room temperature, 18oC to 25oC 4 hours
Refrigerated, 2oC to 8oC 4 hours
Frozen, -20oC 2 weeks
Frozen, -70oC 1 year
 
 
 
Test Code:
AT3

Order Name:
Antithrombin III

 
Method detail:
Chromogenic substrate
 
Schedule:
Tested every Tuesday and Friday.
Note: If not delivered at the time of testing, will be done in the next round
 
Turnaround Time:
Collected specimen to reported within 4 days
 
Performing Location:
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time:
14 days
 
 
 
Test Code:
AT3

Order Name:
Antithrombin III

 
 
Clinical Information:
Antithrombin (AT) or Heparin Cofactor I is the major inhibitor of blood coagulation and is essential for effective heparin therapy. By inhibiting the coagulation proteases, especially thrombin, FXa and FIXa, AT prevents uncontrolled coagulation and thrombosis. Antithrombin deficiency is associated with a high risk of thromboembolic disorders.

The Liquid Antithrombin kit is an assay based on a synthetic chromogenic substrate and on FXa inactivation Antithrombin levels in patient plasma are measured automatically in two stages:
  1. Incubation of the plasma with the Factor Xa reagent in the presence of an excess of heparin.
  2. Quantification of the residual FXa activity with a synthetic chromogenic substrate.  The paranitroaniline released is monitored kinetically at 405 nm and is inversely proportional to the Antithrombin level in the test sample.
 
Reference Value:
83-128 % Activity
 
Clinical Reference:
  1. Manufacturer’s reagent Package Insert, Liquid Antithrombin HemosIL®, Instrumentation Laboratory Company - Bedford, MA 01730-2443 (USA), Instrumentation Laboratory SpA- V.le Monza 338 - 20128 Milano (Italy), 08/2014
  2. https://www.mayocliniclabs.com (Retrieved: 22 Jan 2019)