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Test Code:
ANCAEP

Order Name:
ANCA Profile for Inflammatory Bowel Disease (IBD)

 
Useful For:
Diagnosis of autoimmune diseases like Wegener's granulomatosis, rapid progressive glomerulonephritis, polyarteritis, ulcerative colitis, primary sclerosing cholangitis in the suspected patient.
 
Methodology:
Enzyme-linked Immunosorbent Assay (ELISA)
 
Test List In Profile:
cANCA/Anti-Proteinase 3 
pANCA/Anti-Lactoferrin  
pANCA/Anti-Myeloperoxidase 
pANCA/Anti-Elastase   
pANCA/Anti-Cathepsin G  
pANCA/Anti-BPI   
 
AliasesName:
ANCA (Antineutrophil Cytoplasmic Antibodies)
Anti-Myeloperoxidase Antibodies
Myeloperoxidase Antibodies (MPO)
Antibodies to Myeloperoxidase
Antineutrophil Cytoplasmic Antibodies (ACPA)
Neutrophil Cytoplasmic Antibodies
Autoantibodies to Myeloperoxidase
Autoantibodies to Proteinase 3
Cytoplasmic Neutrophil Antibodies
Perinuclear Antineutrophil Cytoplasmic Antibody (pANCA)
 
 
 
Test Code:
ANCAEP

Order Name:
ANCA Profile for Inflammatory Bowel Disease (IBD)

 
Collection Specimen Or Container:
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 0.5 mL
 
Sub Mission Container:
Plastic tube
 
Rejection Criteria:
Hemolysis: Mild OK; Gross reject
Lipemia: Mild OK; Gross reject
Icterus: Mild OK; Gross reject
Other: N/A
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 14 days
 
 
 
Test Code:
ANCAEP

Order Name:
ANCA Profile for Inflammatory Bowel Disease (IBD)

 
Method detail:
Enzyme-linked Immunosorbent Assay (ELISA)
 
Schedule:
Test daily, at 01:00 p.m.
 
Turnaround Time:
Received specimen to reported within 2 days.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
ANCAEP

Order Name:
ANCA Profile for Inflammatory Bowel Disease (IBD)

 
 
Clinical Information:
Serological testing for neutrophil granulocyte cytoplasmic antibodies (ANCA) is an important aid in the diagnosis of autoimmune diseases like Wegener's granulomatosis, rapid progressive glomerulonephritis, polyarteritis, ulcerative colitis, primary sclerosing cholangitis. ANCA are autoantibodies directed against antigens found in cytoplasmic granules of neutrophils and monocytes. ANCA are classified according to the immunfluorescence patterns they produce on normal neutrophils and according to their target antigens. At least two fluorescence patterns can be differentiated: a granular fluorescence which is distributed regularly over the entire cytoplasm of the granulocytes, leaving the cell nuclei free (cANCA: cytoplasmic pattern), and a predominantly smooth, partly fine granular fluorescence wrapped ribbon-like around the cell nuclei of the granulocytes (pANCA: perinuclear pattern). The cANCA pattern is created by antibodies to proteinase 3. As target antigens of pANCA lactoferrin, myeloperoxidase, elastase, cathepsin G, lysozyme and Beta-glucuronidase has been identified up to now.
 
Reference Value:
Test Reference Value
cANCA/Anti-Proteinase 3 Negative
pANCA/Anti-Lactoferrin Negative
pANCA/Anti-Myeloperoxidase Negative
pANCA/Anti-Elastase Negative
pANCA/Anti-Cathepsin G Negative
pANCA/Anti-BPI Negative
 
Interpretation:
cANCA exhibit a high sensitivity and specificity for Wegener's granulomatosis (prevalence 80 to 95%). The antibody titre correlates with the disease activity. Moreover, cANCA can occur in rare cases in microscopic arteritis and polyarteritis nodosa. The main target antigen of cANCA is proteinase 3. Autoantibodies against lactoferrin, myeloperoxidase, elastase and cathepsin G are associated with various autoimmune diseases like systemic lupus erythematodes, rheumatoid arthritis, Felty's syndrome, rapid progressive glomerulonephritis, microscopic polyarteritis, polyarteritis nodosa, Crohn's disease and ulcerative colitis. Autoantibodies against BPI were described in various diseases among them Wegener's granulomatosis, microscopic polyarteritis, colitis ulcerosa, Crohn's disease, vasculitis, autoimmune hepatitis. Antibodies against granulocytes (pANCA) are not only significant in the serological diagnosis of various forms of vasculitis, but also in the differential diagnosis of chronic inflammatory bowel disease. pANCA occurs in 67% of patients with ulcerative colitis and can also occur in Crohn's disease (7%).
 
Clinical Reference:
Manufacturer’s reagent package insert, ANCA Profile ELISA (IgG) Test instruction, September 2016, EUROIMMUN, Lübeck, D-23560 Germany.