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Test Code:
25OHVDTT

Order Name:
25-OH Vitamin D, Total

 
Useful For:
  • Determination of 25-hydroxyvitamin D (25-OH vitamin D) in human serum.
  • Diagnosis of vitamin D deficiency
  • Differential diagnosis of causes of rickets and osteomalacia 
  • Monitoring vitamin D replacement therapy
  • Diagnosis of hypervitaminosis D
 
Methodology:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
AliasesName:
25-Hydroxyvitamin D2 and D3
 
 
 
Test Code:
25OHVDTT

Order Name:
25-OH Vitamin D, Total

 
Collection Specimen Or Container:
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 2 mL
 
Sub Mission Container:
Plastic vial
 
Rejection Criteria:
Hemolysis: 4+ reject.
Lipemia: 4+ reject.
Icterus: 4+ reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum (in original tube) Room temperature, 18oC to 24oC 8 hours
Serum Room temperature, 18oC to 24oC 72 hours
Refrigerated, 2oC to 8oC 12 days
Frozen, -20oC 12 months
 
 
 
Test Code:
25OHVDTT

Order Name:
25-OH Vitamin D, Total

 
Method detail:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Specimen collected to reported within 2:00 Hours (120 Mins)
 
Performing Location:
Immunology, Laboratory Department Tel. 13227
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
25OHVDTT

Order Name:
25-OH Vitamin D, Total

 
 
Clinical Information:
Vitamin D is a fat-soluble steroid prohormone mainly produced photochemically in the skin from 7-dehydrocholesterol. Two forms of vitamin D are biologically relevant - vitamin D3 (Cholecalciferol) and vitamin D2 (Ergocalciferol). Both vitamins D3 and D2 can be absorbed from food, with vitamin D2 being an artificial source, but only an estimated 10-20% of vitamin D is supplied through nutritional intake. 

Vitamins D3 and D2 can be found in vitamin supplements. Vitamin D is converted to the active hormone 1,25-(OH)2-vitamin D (Calcitriol) through two hydroxylation reactions. The first hydroxylation converts vitamin D into 25-OH vitamin D and occurs in the liver. The second hydroxylation converts 25-OH vitamin D into the biologically active 1,25-(OH)2-vitamin D and occurs in the kidneys as well as in many other cells of the body. Most cells express the vitamin D receptor and about 3% of the human genome is directly or indirectly regulated by the vitamin D endocrine system.

The major storage form of vitamin D is 25-OH vitamin D and is present in the blood at up to 1,000 fold higher concentration compared to the active 1,25-(OH)2-vitamin D. 25-OH vitamin D has a half-life of 2-3 weeks vs. 4 hours for 1,25-(OH)2-vitamin D. Therefore, 25-OH vitamin D is the analyte of choice for determination of the vitamin D status. Epidemiological studies have shown a high global prevalence of vitamin D insufficiency and deficiency. Risk factors for vitamin D deficiency include low sun exposure, malnutrition, some malabsorption syndromes, and liver or kidney diseases. The measurement of vitamin D status provides opportunities for
preventive and therapeutic interventions. Vitamin D deficiency is a cause of secondary hyperparathyroidism
and diseases resulting in impaired bone metabolism (like rickets, osteoporosis, osteomalacia) 
 
Reference Value:
  Concentration in serum (ng/mL)
Optimal >= 30
Insufficiency 21 - 29
Deficiency <= 20
Toxicity possible > 100
 
Clinical Reference:
  1. Manufacturer’s Reagent package insert, 25-OH Vitamin D, August2016, Abbott Ireland Diagnostic Division Lisnamuck, Longford Co. Longford, Ireland.
  2. (*) Reference range: Reference range from The Endocrine Society.
  3. (*) Holick MF et al. Evaluation, treatment and prevention of vitamin D defieciency: an endocrine society clinical practice guideline. J Clin Endocrine Metab 2011. 96(7) 1911-1930
  4. http://www.mayomedicallaboratories.com (Retrieved: 01 Jun 2020)