Pneumonia Panel by FilmArray, PCR

1. Nasopharyngeal swab and Throat swab/ Universal Transport Media (UTM) 3 mL, 1 tube or
2. Bronchoalveolar lavage (BAL)/Sterile Container or
3. Bronchial washing/Sterile Container or
4. Sputum/Sterile Container

1. Nasopharyngeal swab and Throat swab/ Universal Transport Media (UTM) 3 mL, 1 tube or
2. Bronchoalveolar lavage (BAL), 5-10 mL (Minimum volume 1.5 mL)
3. Bronchial washing, 5-10 mL (Minimum volume 1.5 mL)
4. Sputum, 2-3 mL (Minimum volume 0.5 mL)

Universal Transport Media (UTM) 
Sterile Container

1. Specimen is added any preservative solution
2. Sputum is mostly saliva not true sputum.
3. Specimen is centrifuged, diluted, or otherwise processed prior to testing. 

Pneumonia Panel by FilmArray, PCR

24 hours service

3 hours
(Specimen received to reported)

Molecular biology, Laboratory Department Tel.14168

7 days

Pneumonia Panel by FilmArray, PCR

Pathogens infecting the lower respiratory tract cause acute local and systemic disease, with the most severe cases occurring in children, the elderly, and immunocompromised individuals. Lower respiratory symptoms can include shortness of breath, weakness, high fever, coughing, and fatigue. Due to the similarity of diseases caused by many viruses and bacteria, diagnosis based on clinical symptoms alone is difficult. Identification of potential causative agents, as well as the relative abundance of common bacterial agents, provides data to aid the physician in determining appropriate patient treatment and public health response for disease containment.

Acinetobacter calcoaceticusbaumannii complex	Not Detected
Enterobacter cloacae complex	Not Detected
Escherichia coli	Not Detected
Haemophilus influenzae	Not Detected
Klebsiella aerogenes	Not Detected
Klebsiella oxytoca	Not Detected
Klebsiella pneumoniae group	Not Detected
Moraxella catarrhalis	Not Detected
Proteus spp.	Not Detected
Pseudomonas aeruginosa	Not Detected
Serratia marcescens	Not Detected
Staphylococcus aureus	Not Detected
Streptococcus agalactiae	Not Detected
Streptococcus pneumoniae	Not Detected
Streptococcus pyogenes	Not Detected
Chlamydia pneumoniae	Not Detected
Legionella pneumophila	Not Detected
Mycoplasma pneumoniae	Not Detected
Adenovirus	Not Detected
Coronavirus	Not Detected
Human Metapneumovirus	Not Detected
Human Rhinovirus/Enterovirus	Not Detected
Influenza A	Not Detected
Influenza B	Not Detected
Middle East Respiratory Syndrome Coronavirus (MERS-CoV)	Not Detected
Parainfluenza Virus	Not Detected
Respiratory Syncytial Virus	Not Detected

 

1. Viral and bacterial nucleic acids may persist in vivo independent of organism viability. Detection of organism target(s) does not imply that the corresponding organisms are infectious or are the causative agents for clinical symptoms. 
2. The BIOFIRE Pneumonia Panel results for bacteria are provided as a qualitative Detected/Not Detected result with an associated semi-quantitative bin result of 10^4, 10^5, 10^6, or ≥10^7 copies of genomic nucleic acid per milliliter of specimen. An exact quantitative value is not provided. The semi-quantitative (copies/mL) bin result does not distinguish between nucleic acid from live or dead bacteria.  
3. A negative BIOFIRE Pneumonia Panel result does not exclude the possibility of viral or bacterial infection. Negative test results may occur from the presence of sequence variants in the region targeted by the assay, the presence of inhibitors, sample mix-up, or an infection caused by an organism not detected by the panel. Test results may also be affected by concurrent antiviral/antibacterial therapy or levels of organism in the specimen that are below the limit of detection for the test or below the reportable level for bacterial analytes. Negative results should not be used as the sole basis for diagnosis, treatment, or other patient management decisions.  
4. Concomitant culture of specimens is required with the BIOFIRE Pneumonia Panel. Culture is needed for recovery of isolates and antimicrobial susceptibility testing, as well as further speciation of genus, complex, or group level results.  
5. Due to the genetic similarity between human rhinovirus and enterovirus, the BIOFIRE Pneumonia Panel cannot reliably differentiate them. A BIOFIRE Pneumonia Panel Human Rhinovirus/Enterovirus Detected result should be followed-up using an alternate method (e.g. cell culture or sequence analysis) if differentiation between the viruses is required. 
6. False positives and false negatives can be the result of a variety of sources and causes. It is important that results be used in conjunction with other clinical, epidemiological, or laboratory information. 

Manufacturer’s package inserts. FilmArray Pneumonia Panel plus, November 2024, BioFire Diagnostics, Salt Lake City, UT 84108, USA.