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Clear
 
Test Code:
090-71-0501-02

Order Name:
PCR for Lyme Disease ( CSF or Synovial fluid ) **

 
Useful For:

Supporting the diagnosis of Lyme disease in conjunction with serologic testing
Specific indications including testing synovial fluid or synovium to support the diagnosis of Lyme arthritis


 
 
Methodology:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
 
Test List In Profile:
B. burgdorferi PCR
B. mayonii PCR
B. garinii/B. afzelii PCR
 
AliasesName:

Lyme Disease, Molecular Detection, PCR, CSF or Synovial fluid

 
 
 
Test Code:
090-71-0501-02

Order Name:
PCR for Lyme Disease ( CSF or Synovial fluid ) **

 
Patient Preparation:
N/A
 
Collection Specimen Or Container:
CSF or Synovial fluid/ Sterile container

ระบุชนิดสิ่งส่งตรวจ ( Specimen source is required, please specify )
เจาะเก็บสิ่งส่งตรวจ วันอังคารหรือวันพุธก่อนเที่ยงเท่านั้น และงดวันหยุดนักขัตฤกษ์
 
 
Specimen Testing Type:
CSF or Synovial fluid, minimum volume 1 mL
 
 
Sub Mission Container:
Sterile container
 
Rejection Criteria:
Over stability when reach performing Lab at USA, Severe clotted specimen, Use heparin as anticoagulant
 
Specimen Stabillity:
Specimen Type Temperature Time
CSF or Synovial fluid Refrigerated, 2oC to 8oC (preferred) 7 days **
CSF or Synovial fluid Frozen  7 days **

** Sending to USA
 
 
 
Test Code:
090-71-0501-02

Order Name:
PCR for Lyme Disease ( CSF or Synovial fluid ) **

 
Method detail:
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
 
Schedule:
N/A **Sent out to MAYO, USA
 
Turnaround Time:
Received specimen to reported within 14 days

 
 
Performing Location:
MAYO Laboratory
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code:
090-71-0501-02

Order Name:
PCR for Lyme Disease ( CSF or Synovial fluid ) **

 
 
Clinical Information:
Lyme disease is a multisystem and multistage tick-transmitted infection caused by spirochetal bacteria in the Borrelia burgdorferi sensu lato (Bbsl) complex.(1) Nearly all human infections are caused by 3 Bbsl species; B burgdorferi sensu stricto (hereafter referred to as B burgdorferi) is the primary cause of Lyme disease in North America, while B afzelii and B garinii are the primary causes of Lyme disease in Europe. In 2012, B mayonii was identified as a less common cause of Lyme disease in the upper Midwestern United States.(2,3) This organism has only been detected in patients with exposure to ticks in Minnesota and Wisconsin and has not been detected in over 10,000 specimens from patients in other states including regions of the northeast where Lyme disease is endemic.
 
Reference Value:
Negative
 
Interpretation:

A positive result indicates the presence of DNA from Borrelia burgdorferiB mayoniiB afzelii, or B garinii, the main agents of Lyme disease.

A negative result indicates the absence of detectable target DNA in the specimen. Due to the clinical sensitivity limitations of the PCR assay, a negative result does not preclude the presence of the organism or active Lyme disease.

 
Clinical Reference:
www.mayocliniclabs.com (Retrieved: 7 Jul 2020)