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Overview
ข้อมูลทั่วไป Specimen
ข้อมูลสิ่งส่งตรวจ Performance
ข้อมูลการทดสอบ Clinical & Interpretation
ข้อมูลทางคลินิก
 
Test Code (รหัสการทดสอบ):
090-70-0041
Order Name (ชื่อการทดสอบ):
EBV (Epstein-Barr Virus), PCR
 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
1. Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube or
2. Bone marrow/ K3 EDTA (Lavender Top) 3 mL, 1 tube or
3. CSF/ Sterile Container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 4 days.
 
Useful For (ประโยชน์การทดสอบ):
This test is used to detect EBV virus in whole blood and various other samples and in certaincases is also useful to confirm an EBV primary infection, or to diagnose Hodgkin’s lymphoma or nasopharyngeal carcinoma.
 
Methodology (วิธีการทดสอบ):
Real Time Polymerase chain reaction (Real-Time PCR)
 
AliasesName (ชื่อเรียกอื่นๆ) :
EBV DNA PCR
EBV PCR
Epstein-Barr virus
Infectious mononucleosis
PCR for EBV
 
 
 
Test Code (รหัสการทดสอบ):
090-70-0041
Order Name (ชื่อการทดสอบ):
EBV (Epstein-Barr Virus), PCR
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
1. Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube or
2. Bone marrow/ K3 EDTA (Lavender Top) 3 mL, 1 tube or
3. CSF/ Sterile Container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
1. Plasma EDTA, minimum volume 1.5 mL
2. Bone marrow, minimum volume 2 mL
3. CSF, minimum volume 1.2 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube or Sterile Container
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: N/A
Lipemia: N/A
Icterus: N/A
Other: Heparin, anticoagulant blood
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Plasma Refrigerated, 2oC to 8oC 1 week
Bone marrow Room temperature, 18oC to 25oC 24 hours
Refrigerated, 2oC to 8oC 48 hours
CSF Room temperature, 18oC to 25oC 24 hours
Refrigerated, 2oC to 8oC 48 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-70-0041
Order Name (ชื่อการทดสอบ):
EBV (Epstein-Barr Virus), PCR
 
Method detail (วิธีการทดสอบ):
Real Time Polymerase chain reaction (Real-Time PCR)
 
Schedule (ตารางการทดสอบ):
Monday and Thursday, at 10:00 a.m.
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 4 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-70-0041
Order Name (ชื่อการทดสอบ):
EBV (Epstein-Barr Virus), PCR
 
 
Clinical Information (ข้อมูลทางคลินิก):
Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that infects the majority of the world population (seroprevalence is greater than 95%). After a primary infection (transmission through contact with saliva), EBV remains latent in B cells. Periodically, EBV can reactivate in immunocompetent individuals. This reactivation is characterized by the production of virions in the oropharynx but is asymptomatic. EBV is the causative agent of infectious mononucleosis. It is also involved in the pathogenesis of several cancers such as Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma. In immunosuppressed individuals, EBV can lead to B-cell lymphoproliferative disorders. Indeed, the main characteristic of EBV is to cause the B lymphocytes to multiply and subsequently produce a malignant lymphoma. In this context, the measurement of the EBV viral load is particularly significant.
 
Reference Value (ค่าอ้างอิง):
Negative
 
Interpretation (การแปลผล):
  1. In healthy individuals can be occasionally detected EBV viremia with no symptoms. Therefore, this test should be used only for patients suspected EBV infection.
  2. It is absolutely necessary to interprete the test results include with other clinical and biological test results in order to define patient viral status.
  3. A negative result does not preclude the presence of EBV infection because the detection of EBV is dependent on the number of organisms present in the specimen and may be affected by specimen collection methods, patient factors and stage of disease.
 
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s package insert, artus® EBV QS-RGQ Kit Handbook, October 2014, Qiagen GmbH, D-40724 Hilden, Germany.