bih.button.backtotop.text
BROWSE BY TEST NAME
%
1
2
3
5
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Clear
 
Test Code:
090-70-0080

Order Name:
PCR for Dengue

 
Useful For:
The assay is intended for the in vitro detection and typing of dengue virus serotypes 1, 2, 3 and 4 in human peripheral blood.
 
Methodology:
Multiplex real-time, reverse transcriptase polymerase chain reaction (RT-PCR) assay
 
AliasesName:
Dengue Fever
Mosquito-borne infection
Dengue, PCR
 
 
 
Test Code:
090-70-0080

Order Name:
PCR for Dengue

 
Collection Specimen Or Container:
Blood/ K3 EDTA (Lavender Top) 3 mL, 2 tubes
 
Specimen Testing Type:
Plasma EDTA, minimum volume 0.5 mL 
Separate plasma within 8 hours of collection.
 
Sub Mission Container:
Plastic tube
 
Rejection Criteria:
Hemolysis: N/A
Lipemia: N/A
Icterus: N/A
Other: Heparin, anticoagulant blood
 
Specimen Stabillity:
Specimen Type Temperature Time
Plasma, EDTA Room temperature 8 hours
Refrigerated, 2oC to 8oC 48 hours
 
 
 
Test Code:
090-70-0080

Order Name:
PCR for Dengue

 
Method detail:
Multiplex real-time, reverse transcriptase polymerase chain reaction (RT-PCR) assay
 
Schedule:
Test daily, at 09:00 a.m.
 
Turnaround Time:
Received specimen to reported within 1 day.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time:
2 months
 
 
 
Test Code:
090-70-0080

Order Name:
PCR for Dengue

 
 
Clinical Information:
Dengue fever (DF) is an acute, self limiting, viral disease that is characterized by fever, headache, body pains, rash, lymphadenopathy, and prostration. In its most severe form, dengue hemorrhagic fever (DHF), infected patients will experience severe fever and renal failure leading to the often fatal dengue shock syndrome (DSS). There are 4 strains of dengue virus, each serologically distinct. Infection with 1 strain does not protect the host from infection by the others. DHF and DSS occur most commonly in individuals that have been infected previously by another strain. The presence of circulating, non-neutralizing, cross-reactive DV antibody may act as an immune infection enhancement factor. Both dengue virus-specific immunoglobulin G (IgG) and IgM antibodies are usually found in the sera from patients with acute primary infections, while the IgM response may be low or sometimes even absent in secondary dengue fever. Furthermore, a strong cross-reactivity exists among the flavivirus family. As a result, the antibody response may be difficult to interpret with regard to an acute dengue fever, if other flavivirus infections cannot be excluded by clinical, laboratory, or epidemiological means. More recently real-time RT-PCR methods have been developed to detect Dengue virus in the patients’ blood. The detection of dengue virus RNA by reverse transcriptase PCR (RT-PCR) in human blood samples is highly indicative of acute dengue fever. Dengue virus can be detected in the blood from patients for approximately the first 5 days of symptoms.
 
Reference Value:
Negative
 
Interpretation:
  1. The detection result is dependant upon proper sample specimen collection, handling, transportation, storage, and preparation, including extraction.
  2. All results from this tests must be correlated with the clinical history, epidemiological data and other data available to the clinician evaluating the patient.
  3. As with other tests, negative results do not rule out dengue virus infections.
  4. False-negative results may occur when the infecting organism has genomic mutations, insertions, deletions, or rearrangements or when performed very early in the course of illness.
  5. False-negative results may occur if inadequate numbers of organisms are present in the specimen due to improper collection, transport or handling.
  6. This test cannot rule out diseases caused by other viral or bacterial pathogens.
 
Clinical Reference:
Manufacturer’s packages insert, SimplexaTM Dengue, Rev.C, April 2012, Focus Diagnostics, CA 90630 Cypress, USA.