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Test Code:
090-70-0031-03

Order Name:
PCR for CMV (Source specify)

 
Useful For:
The test is intended to be used in conjunction with clinical presentation and other laboratory markers in the diagnosis and management of CMV infection in patients at risk for CMV disease.
 
Methodology:
Real Time Polymerase chain reaction (Real-Time PCR)
 
AliasesName:
CMV DNA qualitative
Cytomegalovirus detection by PCR
CMV by PCR
PCR for Cytomegalovirus 
 
 
 
Test Code:
090-70-0031-03

Order Name:
PCR for CMV (Source specify)

 
Collection Specimen Or Container:
  1. Sputum/ Sterile Container or
  2. Bronchial washing/ Sterile Container or
  3. Bronchoalveolar lavage (BAL)/ Sterile Container or
  4. Bone marrow/ K3 EDTA (Lavender Top) 3 mL, 1 tube or
  5. Tissue/ Sterile Container or
  6. Random Urine/ Sterile Container or
  7. Stool/ Clean container or
 
Specimen Testing Type:
  1. Sputum 2-3 mL (Minimum volume = 0.5 mL)
  2. Bronchial washing 10-20 mL (Minimum volume = 1.5 mL)
  3. Bronchoalveolar lavage 10-20 mL (Minimum volume = 1.5 mL)
  4. Bone marrow 3 mL (Minimum volume = 1 mL)
  5. Tissue
  6. Random Urine 20-50 mL (Minimum volume = 5 mL)
  7. Stool 1 mL
 
Sub Mission Container:
Plastic tube/Sterile Container
 
Rejection Criteria:
  1. Heparin, anticoagulant blood
  2. Specimen is added any preservative solution
  3. Plasma is not separated within 36 hours after collection.
  4. Sputum is mostly saliva not true sputum.
  5. Stool is mixed with toilet paper, water, soap or urine.
 
Specimen Stabillity:
Specimen Type Temperature Time
Sputum Refrigerated, 2oC to 8oC 7 days
Bronchial washing
Bronchoalveolar lavage (BAL)
Bone marrow At temperature, 2oC to 25oC 36 hours
Tissue Refrigerated, 2oC to 8oC 7 days
Random Urine At room temperature 1 day
Stool
 
 
 
Test Code:
090-70-0031-03

Order Name:
PCR for CMV (Source specify)

 
Method detail:
Real Time Polymerase chain reaction (Real-Time PCR)
 
Schedule:
Monday and Saturday, at 10:00 a.m. Wednesday, at 3:00 p.m.
 
Turnaround Time:
Received specimen to reported within 3 days.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-70-0031-03

Order Name:
PCR for CMV (Source specify)

 
 
Clinical Information:
Human cytomegalovirus (HCMV or CMV) is a human viral pathogen belonging to the Herpesvirus family found in both the developed industrial societies and in isolated aboriginal groups. It can be transmitted through blood, oropharyngeal secretions, urine, cervical and vaginal excretions, spermatic fluids, breast milk, tears and feces. Primary infections with CMV in immunocompetent individuals are usually asymptomatic and often give rise to undetected latent infections. Peripheral blood mononuclear cells and endothelial cells appear to be the major sites of infection. CMV remains in a latent stage in monocytes/macrophages in humans. Latently infected individuals may intermittently shed the virus in their body fluids and thus infect others. Immunocompromised individuals, including neonates, transplant patients and AIDS patients, are at high risk for developing severe CMV infections that can lead to a higher rate of morbidity and mortality. Severe clinical manifestations of CMV disease include CMV syndrome, retinitis, gastroenteritis, hepatitis, encephalitis, esophagitis, enterocolitis, pancreatitis and pneumonia.
 
Reference Value:
Negative
 
Interpretation:
  1. A result of "Negative" indicates the absence of cytomegalovirus (CMV) DNA in the plasma.
  2. Reliable results are dependent on adequate specimen collection, transport, storage and processing procedures.
  3. Mutations within the highly conserved regions of the viral genome covered by the test primers and/or probes have been identified and may result in under-quantitation of or failure to detect the virus.
  4. Detection of CMV DNA is dependent on the number of virus particles present in the specimen and may be affected by specimen collection methods and patient factors, (ie, age, presence of symptoms, and/or stage of the infection).
 
Clinical Reference:
Manufacturer’s package insert, COBAS® AmpliPrep/COBAS® TaqMan®CMV Test, September 2019, Roche Molecular Systems, NJ 08876 Branchburg, USA.