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Test Code (รหัสการทดสอบ):
CMVPCR

Order Name (ชื่อการทดสอบ):
PCR for CMV (Blood)

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Useful For (ประโยชน์การทดสอบ):
The test is intended to be used in conjunction with clinical presentation and other laboratory markers in the diagnosis and management of CMV infection in patients at risk for CMV disease
 
Methodology (วิธีการทดสอบ):
Real Time Polymerase chain reaction (Real-Time PCR)
 
AliasesName (ชื่อเรียกอื่นๆ) :
CMV DNA qualitative
Cytomegalovirus detection by PCR
CMV by PCR
PCR for Cytomegalovirus 
 
 
 
Test Code (รหัสการทดสอบ):
CMVPCR

Order Name (ชื่อการทดสอบ):
PCR for CMV (Blood)

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ K3 EDTA (Lavender Top) 3 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Plasma EDTA, minimum volume 1 mL 
Separate plasma within 36 hours of collection.
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: N/A
Lipemia: N/A
Icterus: N/A
Other :
  1. Heparin, anticoagulant blood
  2. Plasma is not separated within 36 hours after collection.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Whole blood At temperature, 2oC to 25oC 36 hours
Plasma Refrigerated, 2oC to 8oC 7 days
Frozen, ≤ -20oC 6 weeks
 
 
 
Test Code (รหัสการทดสอบ):
CMVPCR

Order Name (ชื่อการทดสอบ):
PCR for CMV (Blood)

 
Method detail (วิธีการทดสอบ):
Real Time Polymerase chain reaction (Real-Time PCR)
 
Schedule (ตารางการทดสอบ):
Monday and Saturday, at 10:00 a.m. Wednesday, at 3:00 p.m.
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
2 months
 
 
 
Test Code (รหัสการทดสอบ):
CMVPCR

Order Name (ชื่อการทดสอบ):
PCR for CMV (Blood)

 
 
Clinical Information (ข้อมูลทางคลินิก):
Human cytomegalovirus (HCMV or CMV) is a human viral pathogen belonging to the Herpesvirus family found in both the developed industrial societies and in isolated aboriginal groups. It can be transmitted through blood, oropharyngeal secretions, urine, cervical and vaginal excretions, spermatic fluids, breast milk, tears and feces. Primary infections with CMV in immunocompetent individuals are usually asymptomatic and often give rise to undetected latent infections. Peripheral blood mononuclear cells and endothelial cells appear to be the major sites of infection. CMV remains in a latent stage in monocytes/macrophages in humans. Latently infected individuals may intermittently shed the virus in their body fluids and thus infect others. Immunocompromised individuals, including neonates, transplant patients and AIDS patients, are at high risk for developing severe CMV infections that can lead to a higher rate of morbidity and mortality. Severe clinical manifestations of CMV disease include CMV syndrome, retinitis, gastroenteritis, hepatitis, encephalitis, esophagitis, enterocolitis, pancreatitis and pneumonia.
 
Reference Value (ค่าอ้างอิง):
Negative
 
Interpretation (การแปลผล):
  1. A result of "Negative" indicates the absence of cytomegalovirus (CMV) DNA in the plasma.
  2. Reliable results are dependent on adequate specimen collection, transport, storage and processing procedures.
  3. Mutations within the highly conserved regions of the viral genome covered by the test primers and/or probes have been identified and may result in under-quantitation of or failure to detect the virus.
  4. Detection of CMV DNA is dependent on the number of virus particles present in the specimen and may be affected by specimen collection methods and patient factors, (ie, age, presence of symptoms, and/or stage of the infection).
 
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s package insert, COBAS® AmpliPrep/COBAS® TaqMan®CMV Test, September 2019, Roche Molecular Systems, NJ 08876 Branchburg, USA.