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Clear
 
Test Code:
090-40-0780-01

Order Name:
Modified AFB Stain (Source Specify)

 
Useful For:
Detection of acid-fast bacilli in clinical specimens
 
Methodology:
Modified Kinyoun Stain Microscopic
 
AliasesName:
Nocardia Stain
Rhodococcus stain
Special Stain for Fungus
Nocardia Aerobic
Actinomycetes
Cryptosporidium stain
Isospora stain
 
 
 
Test Code:
090-40-0780-01

Order Name:
Modified AFB Stain (Source Specify)

 
Collection Specimen Or Container:
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Tissue/ Sterile container or Microscopic slide
  4. Urine/ Sterile container
  5. Stool/ Sterile container
 
Specimen Testing Type:
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Sputum, Bronchoalveolar lavage, Bronchial wash volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue volume Entire collection (minimum 0.5 mL)
  4. Urine, volume Entire collection (minimum 1 mL)
  5. Stool, volume Entire collection (minimum 1 gram.)
 
Sub Mission Container:
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Specimen Stabillity:
 
Specimen Type Temperature Time
Body fluid Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 15 minutes
Stool Room temperature, 18oC to 25oC 1 hours
Refrigerated, 2oC to 8oC 24 hours
Others specimen Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
 
 
 
Test Code:
090-40-0780-01

Order Name:
Modified AFB Stain (Source Specify)

 
Method detail:
Modified Kinyoun Stain Microscopic
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 3 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14172
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0780-01

Order Name:
Modified AFB Stain (Source Specify)

 
 
Clinical Information:
Nocardia asteroides, the most commonly recognized aerobic actinomycete, causes significant disease in immunocompromised patients. Clinical presentations can include pneumonia, skin abscess, bacteremia, brain abscess, eye infection, and joint infection. Other species associated with human disease include Nocardia brasiliensis, Nocardia otitidiscaviarum, Nocardia farcinica, Nocardia nova, and Nocardia transvalensis. Cryptosporidium and Isospora species have been identified as causes of severe diarrhea in immunocompromised hosts, but they can also cause diarrhea in the immunocompetent host. Oocysts in clinical specimens may be difficult to identify with out special staining.
 
Reference Value:
No Modified AFB, Cryptosporidium and Isospora seen
 
Interpretation:
Positive slides are reported as one or more of the following: - Filamentous and Branching of Nocardia like Microorganism - Cocci of Rhodococcus like Microorganism - Cryptosporidium - Isospora
 
Clinical Reference:
https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)