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Test Code:
090-10-0670-02

Order Name:
Ketone (Dip , Urine)

 
Useful For:
A semiquantitative test, it is used to evaluate ketonuria, detect acidosis, ketoacidosis of alcoholism and diabetes mellitus, fasting, starvation, high protein diets, and isopropanol ingestion. It remains useful as a monitor in known diabetics, in type I patients when ill and during marked hyperglycemia and in type II diabetics during acute illness.
 
Methodology:
This test is based on "Legal's method" in which the test pad contains sodium nitroprusside and glycine in an alkaline medium. A violet color proportional to methylketone is generated.
 
 
 
Test Code:
090-10-0670-02

Order Name:
Ketone (Dip , Urine)

 
Collection Specimen Or Container:
Urine/ Clean container 20 mL
 
Specimen Testing Type:
Urine, minimum volume 2 mL
 
Sub Mission Container:
Clean container
 
Specimen Stabillity:
Specimen Type Temperature Time
Random Urine Room temperature, 18oC to 25oC 2 hours
 
 
 
Test Code:
090-10-0670-02

Order Name:
Ketone (Dip , Urine)

 
Method detail:
This test is based on "Legal's method" in which the test pad contains sodium nitroprusside and glycine in an alkaline medium. A violet color proportional to methylketone is generated.
 
Schedule:
Tested Daily (24 hours)
 
Turnaround Time:
Collected specimen to report within 1 hour
 
Performing Location:
Hematology, Laboratory Department Tel. 17254
 
Specimen Retention Time:
3 days
 
 
 
Test Code:
090-10-0670-02

Order Name:
Ketone (Dip , Urine)

 
 
Clinical Information:
In adult healthy men, a fast of 18 hours or greater produces ketonemia at a level that would result in detectable ketonuria. Aging is associated with increased susceptibility to fasting-induced hyperketonemia. (London, 1986) Ketonuria may be noted in normal pregnancy. (Chez, 1987) Acetoacetic acid, beta-hydroxybutyric acid, and acetone are ketone bodies. In ketosis, usually 80% of total ketones are beta-hydroxybutyric acid. Acetoacetic acid comprises most of the remainder with acetone present in trace amounts. Urine ketones should generally be determined in patients with a positive urine test for urine glucose and followed during the management of diabetes mellitus and ketoacidosis. Ketones can depress the glucose oxidase reaction, providing falsely low results, on some glucose oxidase reagent strips. In pregnancy, the risk of ketosis is increased; all pregnant type I diabetics are advised to monitor urine for ketosis in first morning urine and when blood glucose is >150 mg/dL. A portion of initial assessment for inborn errors of metabolism in infancy and childhood. In infants and children, ketonuria can occur with febrile illnesses and toxic states with marked vomiting or diarrhea. Genetic disorders resulting in ketonuria include propionyl CoA carboxylase deficiency, glycogen storage disease, branched-chain ketonuria, and methylmalonic aciduria.
 
Reference Value:
Negative
 
Clinical Reference:
  1. Manufacturer’s Package insert, iChem® VELOCITY™Strips, A Division of IRIS International, Inc.
  2. http://online.lexi.com (Retrieved: 22 Jan 2019)