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Clear
 
Test Code:
090-40-0770-01

Order Name:
KOH Preparation (Source Specify)

 
Useful For:
Detection of fungi in clinical specimens
 
Methodology:
10% KOH Microscopic
 
AliasesName:
KOH stain
Fungal smear
 
 
 
Test Code:
090-40-0770-01

Order Name:
KOH Preparation (Source Specify)

 
Collection Specimen Or Container:
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Hair, Nail, Skin/ Sterile container or Microscopic slide
  4. Vaginal, urethal discharge/ swab into sterile container with 1 mL. normal saline or Microscopic slide
 
Specimen Testing Type:
  1. CSF, Body fluids volume Entire collection (minimum 0.5 mL)
  2. Sputum, Bronchoalveolar lavage, Bronchial wash volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Hair, Nail, Skin volume Entire collection (minimum 0.5 mL) or Microscopic slide
  4. Vaginal, urethal discharge/ swab Entire collectioninto sterile container with 1 mL. normal saline or Microscopic slide
 
Sub Mission Container:
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Specimen Stabillity:
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash (BW) Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage (BAL) Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Sputum Refrigerated, 2oC to 8oC 24 hours
Stool Refrigerated, 2oC to 8oC 24 hours
Tissue Room temperature, 18oC to 25oC 24 hours
Urine Refrigerated, 2oC to 8oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0770-01

Order Name:
KOH Preparation (Source Specify)

 
Method detail:
10% KOH Microscopic
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 2 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0770-01

Order Name:
KOH Preparation (Source Specify)

 
 
Clinical Information:
Many fungi in the environment cause disease in severely compromised human hosts. Accordingly, the range of potential pathogenic fungi has increased as the number of immunosuppressed individuals (persons with AIDS, patients receiving chemotherapy or transplant rejection therapy, etc) has increased. Few fungal diseases can be diagnosed clinically; most are diagnosed by isolating and identifying the infecting fungus in the clinical laboratory.
 
Reference Value:
No fungal element seen
 
Interpretation:
Positive slides are reported as one or more of the following:
  • Septate hyphae
  • Nonseptate hyphae
  • Spherical yeast cells and short hyphae fragments
  • Dichotomous branching septate hyphae
  • Fragment portions of nonseptate hyphae
  • Budding yeast cell
  • Budding yeast cell with pseudohyphae
  • Budding yeast cell with blastoconidia and pseudohyphae
  • Pseudohyphae
  • Macroconidia
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)