Infections with hepatitis E virus (HEV) are after hepatitis A and B the most frequent cause of hepatitis worldwide. Since the clinical symptoms of hepatitis E resemble those of other hepatitides, laboratory diagnostics play an essential role in diagnosis. Alongside PCR detection of viral RNA in blood or stool, the serological determination of antibodies of classes IgA/IgG/IgM against HEV is the most important aid for diagnosis of an HEV infection. Pathogen-specific antibodies are usually detectable when the first clinical symptoms appear or shortly afterwards. A positive IgA and/or IgM test and a significant increase in IgG in a serum pair (taken at a time interval of 8-14 days) indicate an acute infection. IgA and IgM anti-HEV titers usually drop rapidly after infection, while the IgG HEV titer persists for over 10 years.
Manufacturer’s reagent package insert, Anti-Hepatitis E Virus (HEV) ELISA (IgG), Order No. EI 2525G_A_UK_C04.doc, EUROIMMUN, Medizinische Labordiagnostika AG. Version:08/08/2018