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Test Code:
MIBTS0040

Order Name:
Gram Stain (Vagina)

 
Useful For:
Supporting the diagnosis of bacterial vaginosis
 
Methodology:
Conventional Gram Stain Procedure and Nugent Scoring System
 
AliasesName:
Gram stain Gram Vagina
 
 
 
Test Code:
MIBTS0040

Order Name:
Gram Stain (Vagina)

 
Collection Specimen Or Container:
Vaginal swab/ Slide container
 
Specimen Testing Type:
Smear on Microscopic Slide
 
Sub Mission Container:
Slide container
 
Rejection Criteria:
Swab in Stuart transport medium will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Vaginal smear Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
MIBTS0040

Order Name:
Gram Stain (Vagina)

 
Method detail:
Conventional Gram Stain Procedure and Nugent Scoring System
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 2 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
1 month
 
 
 
Test Code:
MIBTS0040

Order Name:
Gram Stain (Vagina)

 
 
Clinical Information:
Bacterial vaginosis is so-named because bacteria are the cause and an associated inflammatory response is lacking. It results in an increase in thin, gray, homogeneous vaginal discharge and vaginal malodor and is caused by a change in the vaginal flora. Bacterial vaginosis is a synergistic polymicrobial infection not caused by a specific organism. The standard scoring system termed the "Nugent score" is a technique for assessing bacterial vaginosis using microscopic examination of a Gram-stained smear of vaginal discharge.
 
Reference Value:
One of the 3 following reports dependent on the weighted sum balance of Lactobacillus, Gardnerella/Bacteroides, and Mobiluncus species:
  1. Consistent with normal bacterial vaginal flora.
  2. Altered vaginal flora not consistent with bacterial vaginosis. This frequently represents a intermediate stage. If signs or symptoms persist, repeat testing is warranted.
  3. Consistent with bacterial vaginosis.
 
Interpretation:
Assessment of a Gram-stained slide using the Nugent score has replaced culture as the preferred test to diagnose bacterial vaginosis. While Gardnerella is the most common anaerobe found in bacterial vaginosis, other anaerobic organisms are often present along with a decrease in the amount of "usual flora" (eg, Lactobacillus species). This system uses a 0- to 4-point scale to calculate the weighted sum of the following 3 bacterial morphotypes: Lactobacillus, Gardnerella/Bacteroides, and Mobiluncus species. Total score of 0 to 3 is considered normal. Total score of 4 to 6 is considered intermediate stage Total score of 7-10 is considered Bacterial vaginosis.
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. ashington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)