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Clear
 
Test Code:
090-40-0750-01

Order Name:
Gram Stain (Source Specify)

 
Useful For:
Detect the presence of bacteria and sometimes fungi in a sample taken from the site of a suspected infection.
 
Methodology:
Conventional Gram Stain Procedure
 
AliasesName:
Gram Stain
 
 
 
Test Code:
090-40-0750-01

Order Name:
Gram Stain (Source Specify)

 
Collection Specimen Or Container:
  1. CSF, Body fluids/ Sterile container
  2. Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge / Sterile container or Microscopic slide
 
Specimen Testing Type:
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Bronchoalveolar lavage, Bronchial wash, volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue, volume Entire collection (minimum 0.5 mL)
 
Sub Mission Container:
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Rejection Criteria:
Swab in Stuart transport medium will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0750-01

Order Name:
Gram Stain (Source Specify)

 
Method detail:
Conventional Gram Stain Procedure
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 2 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
Specimen Type Time
Specimen 7 days
Gram stain slide 1 month
 
 
 
Test Code:
090-40-0750-01

Order Name:
Gram Stain (Source Specify)

 
 
Clinical Information:
The Gram stain is one of the simplest, least expensive, and most useful of the rapid methods used to identify preliminary differentiation of microbiological organisms to provide preliminary information concerning the type of organisms present directly from clinical specimens or from growth on culture plates.
 
Reference Value:
No microorganisms seen
 
Interpretation:
Positive Gram stain results usually include a description of what was seen on the slide. This typically includes: Whether the bacteria are Gram-positive (purple) or Gram-negative (red) Shape — round (cocci) or rods (bacilli) Size, relative quantity, and/or arrangement of the bacteria Whether there are bacteria present within other cells (intracellular) Presence white blood cells Fungi (in the form of yeasts or molds) may be seen on a Gram stain and are reported.
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)
  4. https://labtestsonline.org/tests/gram-stain (Retrieved: 16 Sep 2019)