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Clear
 
Test Code (รหัสการทดสอบ):
090-40-0750-01

Order Name (ชื่อการทดสอบ):
Gram Stain (Source Specify)

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
  1. CSF, Body fluids/ Sterile container
  2. Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge / Sterile container or Microscopic slide
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours.
 
Useful For (ประโยชน์การทดสอบ):
Detect the presence of bacteria and sometimes fungi in a sample taken from the site of a suspected infection.
 
Methodology (วิธีการทดสอบ):
Conventional Gram Stain Procedure
 
AliasesName (ชื่อเรียกอื่นๆ) :
Gram Stain
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0750-01

Order Name (ชื่อการทดสอบ):
Gram Stain (Source Specify)

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
  1. CSF, Body fluids/ Sterile container
  2. Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge / Sterile container or Microscopic slide
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Bronchoalveolar lavage, Bronchial wash, volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue, volume Entire collection (minimum 0.5 mL)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Swab in Stuart transport medium will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0750-01

Order Name (ชื่อการทดสอบ):
Gram Stain (Source Specify)

 
Method detail (วิธีการทดสอบ):
Conventional Gram Stain Procedure
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 2 hours.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
Specimen Type Time
Specimen 7 days
Gram stain slide 1 month
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0750-01

Order Name (ชื่อการทดสอบ):
Gram Stain (Source Specify)

 
 
Clinical Information (ข้อมูลทางคลินิก):
The Gram stain is one of the simplest, least expensive, and most useful of the rapid methods used to identify preliminary differentiation of microbiological organisms to provide preliminary information concerning the type of organisms present directly from clinical specimens or from growth on culture plates.
 
Reference Value (ค่าอ้างอิง):
No microorganisms seen
 
Interpretation (การแปลผล):
Positive Gram stain results usually include a description of what was seen on the slide. This typically includes: Whether the bacteria are Gram-positive (purple) or Gram-negative (red) Shape — round (cocci) or rods (bacilli) Size, relative quantity, and/or arrangement of the bacteria Whether there are bacteria present within other cells (intracellular) Presence white blood cells Fungi (in the form of yeasts or molds) may be seen on a Gram stain and are reported.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)
  4. https://labtestsonline.org/tests/gram-stain (Retrieved: 16 Sep 2019)