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Clear
 
Test Code:
090-40-0930-01

Order Name:
Fungal Stain by Wright-Giemsa (Source Specify)

 
Useful For:
Detection of fungi in clinical specimens
 
Methodology:
Microscopic Wright-Giemsa stain
 
AliasesName:
Fungal smear
Fungal stain
Histoplasma capsulatum
Talaromyces
(formerly Penicillium) marneffei
Septate hyphae
 
 
 
Test Code:
090-40-0930-01

Order Name:
Fungal Stain by Wright-Giemsa (Source Specify)

 
Collection Specimen Or Container:
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Tissue/ Sterile container or Microscopic slide
 
Specimen Testing Type:
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Sputum, Bronchoalveolar lavage, Bronchial wash volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue volume Entire collection
 
Sub Mission Container:
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Rejection Criteria:
Swab in Stuart transport medium or dry swab will be reject.
           
 
Specimen Stabillity:
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Sputum Refrigerated, 2oC to 8oC 24 hours
Tissue Room temperature, 18oC to 25oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0930-01

Order Name:
Fungal Stain by Wright-Giemsa (Source Specify)

 
Method detail:
Microscopic Wright-Giemsa stain
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to report within 3 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0930-01

Order Name:
Fungal Stain by Wright-Giemsa (Source Specify)

 
 
Clinical Information:
Many fungi in the environment cause disease in severely compromised human hosts. Accordingly, the range of potential pathogenic fungi has increased as the number of immunosuppressed individuals (persons with AIDS, patients receiving chemotherapy or transplant rejection therapy, etc) has increased. Few fungal diseases can be diagnosed clinically; most are diagnosed by isolating and identifying the infecting fungus in the clinical laboratory.
 
Reference Value:
No fungal element seen
 
Interpretation:
Positive slides are reported as one or more of the following: yeast or hyphae present
  • Septate hyphae
  • Aseptate hyphae
  • Aspergillus like microorganism
  • Binary fission yeasts like Talaromyces (formerly Penicillium) marneffei
  • Round or oval yeast cells, budding yeast cells
  • Pseudohyphae - Budding yeast cells with Pseudohyphae
  • Budding yeast cell - Yeast cell
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)