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Test Code:
E2

Order Name:
Estradiol (E2)*

 
Useful For:
Determination of estradiol in human serum.
 
Methodology:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
 
 
Test Code:
E2

Order Name:
Estradiol (E2)*

 
Collection Specimen Or Container:
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 1 mL
 
Sub Mission Container:
Plastic vial
 
 
Rejection Criteria:
Hemolysis  4+ will be reject.
 
Specimen Stabillity:
Specimen type Temperature Time
Serum (keep in original tube) Room temperature, 20oC to 28oC 8 hours
Serum Refrigreated, 2oC to 8oC 7 days
Frozen, -20oC Over 7 days
 
 
 
Test Code:
E2

Order Name:
Estradiol (E2)*

 
Method detail:
Chemiluminescent Microparticle Immuno Assay (CMIA)
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Specimen collected to reported within 2:00 hours (120 mins)
 
Performing Location:
Immunology, Laboratory Department Tel. 13227
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
E2

Order Name:
Estradiol (E2)*

 
 
Clinical Information:
Estradiol is the most potent natural estrogen in humans. It regulates reproductive function in females, and, with progesterone, maintains pregnancy. Most estradiol is secreted by the ovaries (non-pregnant women), although the testes (in men) and adrenal cortex (in men and women) secrete small amounts. During pregnancy, the placenta produces most of the circulating estradiol Estradiol and estrone interconvert in vivo. In normal non-pregnant women, estradiol synthesized by the ovary is the predominant source of both estrone and estriol.

Virtually all circulating estradiol is protein-bound. Reported association constants for estradiol with sex hormone binding globulin and serum albumin are, respectively, 6.8 x 108 and 6 x 104. One consequence of this binding is that the conditions of any assay for serum estradiol must release this steroid quantitatively from its binding partners. The amount and proportion of protein-bound and free estradiol vary by gender, and with pregnancy and menstrual phase in women. 

Normal estradiol levels are lowest at menses and into the early follicular phase (25-75 pg/mL) and then rise in the late follicular phase to a peak of 200-600 pg/mL just before the LH surge, which is normally followed
immediately by ovulation. As LH peaks, estradiol begins to decrease before rising again during the luteal phase (100-300 pg/mL). If conception does not take place, estradiol falls further to its lowest levels, and menses
begins shortly thereafter. If conception occurs, estradiol levels continue to rise, reaching levels of 1,000-5,000 pg/mL during the first trimester, 5,000-15,000 pg/mL during second trimester, and 10,000-40,000 pg/mL during third trimester.At menopause, estradiol levels remain low. 

Because the ovaries produce most estradiol in normal women, estimation of this hormone is sometimes a gauge of ovarian function.In addition, monitoring estradiol levels is important in evaluating amenorrhea, precocious puberty, the onset of menopause, and infertility in men and women. Monitoring estradiol levels is essential during in vitro fertilization, because the timing of recovery of oocytes depends on follicular development, which in turn depends on the estradiol level.
 
Reference Value:
Test subject Reference range (pg/mL)
Men 11 - 44
Women Follicular phase
Mid-cycle phase
Luteal phase
21 - 251
38 - 649
21 - 312
Postmenopausal female Not on HRT
on HRT
<10 - 28
<10 - 144
Note: HRT is Hormone Replacement Therapy
 
Clinical Reference:
Manufacturer’s Reagent package insert Architect Estradiol, May 2019, Abbott Ireland Diagnostic Division, Lisnamuck, Longford Co.,Longford,Ireland