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Test Code (รหัสการทดสอบ):
090-40-0150-01

Order Name (ชื่อการทดสอบ):
Cryptococcus C/S (Source Specify)

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
CSF/ Sterile container
  1. 1. Disinfect site with iodine or chlorhexidine preparation.
  2. 2. Insert a needle with stylet at L3-L4, L4-L5, or L5-S1 interspace.
  3. 3. Upon reaching the subarachnoid space, remove the stylet and collect 1–2 ml of fluid into each of three leakproof containers
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 days.
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of Cryptococcal infections
 
Methodology (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
 
AliasesName (ชื่อเรียกอื่นๆ) :
Cryptococcus gattii
Cryptococcus neoformans
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0150-01

Order Name (ชื่อการทดสอบ):
Cryptococcus C/S (Source Specify)

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Medications: Do not use antibiotics before collection of specimen
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
CSF/ Sterile container
  1. 1. Disinfect site with iodine or chlorhexidine preparation.
  2. 2. Insert a needle with stylet at L3-L4, L4-L5, or L5-S1 interspace.
  3. 3. Upon reaching the subarachnoid space, remove the stylet and collect 1–2 ml of fluid into each of three leakproof containers
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
CSF, minimum volume 1 mL.
 
Sub Mission Container (ภาชนะส่งตรวจ):
If only one container of CSF is collected, it should be submitted to microbiology first; otherwise, submit container 2nd to microbiology.
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen in formalin or refrigerated will be rejected.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
CSF Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0150-01

Order Name (ชื่อการทดสอบ):
Cryptococcus C/S (Source Specify)

 
Method detail (วิธีการทดสอบ):
  1. Conventional Culture Technique
  2. Identification by MALDI-TOF
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
1 month
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0150-01

Order Name (ชื่อการทดสอบ):
Cryptococcus C/S (Source Specify)

 
 
Clinical Information (ข้อมูลทางคลินิก):
Cryptococcosis is an invasive fungal infection caused by Cryptococcus neoformans or C gattii. C neoformans has been isolated from several sites in nature, particularly weathered pigeon droppings. C gatti was previously only associated with tropical and subtropical regions; however, more recently this organism has also been found to be endemic in British Columbia and among the Pacific Northwest United States, and is associated with several different trees species.
 
Reference Value (ค่าอ้างอิง):
No Cryptococcal growth
 
Interpretation (การแปลผล):
Cryptococcal meningitis (CM) is a central nervous system infectious disease caused by Cryptococcus. It is the most common fungal infection in the central nervous system, accounting for about 48% of fungal infection. The disease occurs mainly in acquired immunodeficiency syndrome (AIDS) patients and concentrates in the immunocompromised people without AIDS
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s package insert, CALAS, Meridian Diagnostic, USA 2. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)
  2. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  3. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015