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Clear
 
Test Code (รหัสการทดสอบ):
090-40-1040-01

Order Name (ชื่อการทดสอบ):
Cryptococcus Ag (CSF) *

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
CSF/ Sterile container
  1. Disinfect site with iodine or chlorhexidine preparation.
  2. Insert a needle with stylet at L3-L4, L4-L5, or L5-S1 interspace.
  3. Upon reaching the subarachnoid space, remove the stylet and collect 1–2 ml of fluid into each of three leakproof containers
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 hours.
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of cryptococcosis
 
Methodology (วิธีการทดสอบ):
Latex Agglutination
 
Test List In Profile (การทดสอบใน Profile):
CSF C/S or Cryptococcus C/S must be performed with Cryptococcus Ag (CSF)
 
AliasesName (ชื่อเรียกอื่นๆ) :
Cryptococcus Ag Screen,
Serum Cryptococcus gattii
Cryptococcus neoformans
Fungal Serology
 
 
 
Test Code (รหัสการทดสอบ):
090-40-1040-01

Order Name (ชื่อการทดสอบ):
Cryptococcus Ag (CSF) *

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
N/A
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
CSF/ Sterile container
  1. Disinfect site with iodine or chlorhexidine preparation.
  2. Insert a needle with stylet at L3-L4, L4-L5, or L5-S1 interspace.
  3. Upon reaching the subarachnoid space, remove the stylet and collect 1–2 ml of fluid into each of three leakproof containers
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
CSF, minimum volume 1 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
If only one container of CSF is collected, it should be submitted to microbiology first; otherwise, submit container 2nd to microbiology.
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
CSF Room temperature, 18oC to 25oC 15 minutes
 
 
 
Test Code (รหัสการทดสอบ):
090-40-1040-01

Order Name (ชื่อการทดสอบ):
Cryptococcus Ag (CSF) *

 
Method detail (วิธีการทดสอบ):
Latex Agglutination
 
Schedule (ตารางการทดสอบ):
Tested daily
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to report within 3 hours.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
1 month
 
 
 
Test Code (รหัสการทดสอบ):
090-40-1040-01

Order Name (ชื่อการทดสอบ):
Cryptococcus Ag (CSF) *

 
 
Clinical Information (ข้อมูลทางคลินิก):
Cryptococcosis is a systemic infection caused by the yeast, C. neoformans. The natural reservoirs for C. neoformans are soil and avian feces. Inhalation of yeast cells may lead to a lung infection and possibly disseminated disease. Patients often present with devastating debilitation, especially those with an immunosuppressive syndrome. Because C. neoformans has an unusual affinity for central nervous system tissue, rapid and early detection is essential. In 1963, Bloomfield reported detection of cryptococcal polysaccharide (CPS)antigens in serum and CSF of patients with cryptococcosis. In further testing, investigators reported false-positive reactions in the serum of patients with rheumatoid factor. In 1983, Stockman and Roberts introduced an enzymatic method incorporating protease to eliminate interference factors (e.g., rheumatoid factor) in serum. Such factors also include immune complexes, which have been shown to mask CPS antigen and prevent its recognition by sensitized latex particles. Since then, latex agglutination has been found to be a specific and sensitive method for rapid detection of CPS antigen in serum and CSF.
 
Reference Value (ค่าอ้างอิง):
Negative
 
Interpretation (การแปลผล):
A positive result indicates the presence of CPS antigen in the patient specimen. A negative result indicates the absence of detectable levels of CPS antigen in the specimen.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s package insert, CALAS, Meridian Diagnostic, USA
  2. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  3. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015