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Clear
 
Test Code:
090-40-0740-02

Order Name:
Concentration Method for AFB Stain

 
Useful For:
Detection of acid-fast bacilli in sputum
 
Methodology:
  1. The concentration method
  2. The Kinyoun stain method
 
AliasesName:
Acid-Fast Bacilli (AFB)
Acid-Fast Smear for Mycobacterium
AFB (Acid-Fast Bacilli)
Bacillus, Acid-Fast
MTB (Mycobacterium tuberculosis)
Mycobacterium tuberculosis (MTB)
Smear, Acid-Fast Bacilli (AFB)
TB (Tuberculosis)
Tubercle Bacilli: Mycobacterium tuberculosis
Tuberculosis (TB)
 
 
 
Test Code:
090-40-0740-02

Order Name:
Concentration Method for AFB Stain

 
Patient Preparation:
Specimen: Sputum 
  1. Have patient rinse or gargle with water to remove excess oral biota. 
  2. Instruct patient to cough deeply to produce a lower respiratory specimen (not postnasal fluid)
  3. Collect in a sterile container
 
Collection Specimen Or Container:
Sputum/ Sterile container 
 
 
Specimen Testing Type:
Sputum volume Entire collection (minimum 3 mL)
 
Sub Mission Container:
 Sterile container
 
Rejection Criteria:
Specimen not refrigerate >24 hours old 
 
Specimen Stabillity:
Specimen Type Temperature Time
Sputum Room temperature, 18oC to 25oC 2 hours
Refrigerated, 2oC to 8oC 24 hours
 
 
 
Test Code:
090-40-0740-02

Order Name:
Concentration Method for AFB Stain

 
Method detail:
  1. The Sputum concentration method
  2. The Kinyoun stain method
 
Schedule:
Tested daily, at 8:00 a.m. and 11:00 a.m.
 
Turnaround Time:
Received specimen to report within 24 hours.
 
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0740-02

Order Name:
Concentration Method for AFB Stain

 
 
Clinical Information:
Mycobacterium tuberculosis is a leading infectious disease cause of death worldwide. The Centers for Disease Control and Prevention has reported a rise in the incidence of tuberculosis associated with AIDS, foreign-born cases, and increased transmission in high-risk populations. There has also been a rise in the number of M. tuberculosis strains that exhibit resistance to one or more antituberculosis drugs. The public health implications of these facts are considerable. Because M. tuberculosis is readily spread by airborne particles, rapid diagnosis and isolation of infected persons is important. Nontuberculous mycobacteria infections also cause significant morbidity and mortality in humans, particularly in immunocompromised persons. Detection of acid-fast bacilli in sputum specimens allows rapid identification of individuals who are likely to be infected with mycobacteria while definitive diagnosis and treatment are pursued.
 
Reference Value:
No AFB observed
 
Interpretation:
Patients whose sputum specimens are identified as acid-fast positive should be considered potentially infected with Mycobacterium tuberculosis, pending definitive diagnosis by molecular methods or mycobacterial culture.
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)