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Test Code:
CA

Order Name:
Calcium

 
Useful For:
Diagnosis and monitoring of a wide range of disorders including diseases of bone, kidney, parathyroid gland, or gastrointestinal tract
 
Methodology:
Arsenazo III
 
AliasesName:
Ca (Calcium), Blood
 
 
 
Test Code:
CA

Order Name:
Calcium

 
Collection Specimen Or Container:
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type:
Serum, minimum volume 0.5 mL
 
Sub Mission Container:
Plastic vial
 
Rejection Criteria:
Hemolysis: 4+ reject
 
 
Specimen Stabillity:
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 2 days
Frozen, -20oC 8 months
 
 
 
Test Code:
CA

Order Name:
Calcium

 
Method detail:
Arsenazo III
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Collected specimen to report within 1.5 hours (90 mins)
 
Performing Location:
Chemistry, Laboratory Department Tel. 13224
 
Specimen Retention Time:
5 days
 
 
 
Test Code:
CA

Order Name:
Calcium

 
 
Clinical Information:
The majority of calcium in the body is present in bones. The remainder of the calcium is in serum and has various functions. For example, calcium ions decrease neuromuscular excitability, participate in blood coagulation, and activate some enzymes.

Hypercalcemia can result from hyperparathyroidism, hypervitaminosis D, multiple myeloma, and some neoplastic diseases of bone. Long-term lithium therapy has been reported to cause hyperparathyroidism in some individuals, with resulting hypercalcemia.

Hypocalcemia can result from hypoparathyroidism, hypoalbuminemia, renal insufficiency, and pancreatitis.

Calcium has traditionally been difficult to measure accurately and precisely, and a large variety of methods have been developed. Among these are flame photometry, oxalate precipitation with titration, atomic absorption spectrophotometry, EDTA chelation, and more recently calcium dye complexes which are measured spectrophotometrically.

Examples of calcium dyes are o-cresolphthalein complexone and Arsenazo III, the latter being the dye used for calcium determination in this method.
 
Reference Value:
8.4 – 10.2 mg/dL
 
Interpretation:
Hypocalcemia:
Long-term therapy must be tailored to the specific disease causing the hypocalcemia. The therapeutic endpoint is to achieve a serum calcium level of 8.0 to 8.5 mg/dL to prevent tetany. For symptomatic hypocalcemia, calcium may be administered intravenously.

Hypercalcemia:
The level at which hypercalcemic symptoms occur varies from patient to patient. Symptoms are common when serum calcium levels are above 11.5 mg/dL, although patients may be asymptomatic at this level. Levels above 12.0 mg/dL are considered a critical value in the Mayo Health System. Severe hypercalcemia (>15.0 mg/dL) is a medical emergency.
 
Clinical Reference:
  1. Manufacturer’s reagent package insert, Architect® Calcium, ABBOTT Laboratories, Abbott Park IL 60064 USA, January 2016.
  2. http://www.mayomedicallaboratories.com (Retrieved: 01 Jan 2019)