bih.button.backtotop.text
BROWSE BY TEST NAME
%
1
2
3
5
8
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Clear
 
Test Code (รหัสการทดสอบ):
090-70-8505

Order Name (ชื่อการทดสอบ):
ANA Profile II by Immunoblot

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 2 days
 
Useful For (ประโยชน์การทดสอบ):
Diagnosis of a systemic rheumatic disease in the suspected patient.
 
Methodology (วิธีการทดสอบ):
Immunoblot
 
Test List In Profile (การทดสอบใน Profile):
Anti nRNP/Sm
Anti Sm
Anti SS-A  
Anti Ro-52
Anti SS-B 
Anti Scl-70 
Anti PM-Scl
Anti Jo-1 
Anti Centromere Protein B (CENP B)
Anti PCNA
Anti dsDNA 
Anti Nucleosomes   
Anti Histones
Anti Ribosomal P-Protein
Anti AMA-M2
Anti DFS70
 
AliasesName (ชื่อเรียกอื่นๆ) :
Anti-Ribonuclear Protein
Autoantibodies to U1RNP
Ribonucleoprotein (RNP) Autoantibodies
Autoantibodies to Sm (Smith)
Sm (Anti-Sm) (Smith)
Sm (Smith) Autoantibodies
Anti-Ro
Anti-Sjogren's Syndrome
Anti-SSA (Anti-Sjogren's Syndrome A)
Autoantibodies to SSA (Ro)
Ro (Anti-SSA)
Anti-La
Anti-SSB (Anti-Sjogren's Syndrome B)
Autoantibodies to SS-B/LA, Serum
La (Anti-SSB)
SSB (Anti-SSB)
ACA (Anticentromere Antibodies)
Anticentromere Antibodies
Centromere Antibodies, IgG
Centromere Autoantibodies
Anti-PM/Scl Antibodies
Mitochondrial Antibodies (M2)
AMA (Antimitochondrial Antibodies)
Antibodies against proliferating cell nuclear antigen
Nuclear dense fine speckled
Anti-LEDGF
 
 
 
 
Test Code (รหัสการทดสอบ):
090-70-8505

Order Name (ชื่อการทดสอบ):
ANA Profile II by Immunoblot

 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Plain blood (Red top) 6 mL, 1 tube
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Serum, minimum volume 0.5 mL
 
Sub Mission Container (ภาชนะส่งตรวจ):
Plastic tube
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis: Mild OK; Gross reject
Lipemia: Mild OK; Gross reject
Icterus: Mild OK; Gross reject
Other: N/A
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Serum Refrigerated, 2oC to 8oC 14 days
 
 
 
Test Code (รหัสการทดสอบ):
090-70-8505

Order Name (ชื่อการทดสอบ):
ANA Profile II by Immunoblot

 
Method detail (วิธีการทดสอบ):
Immunoblot
 
Schedule (ตารางการทดสอบ):
Test daily, at 12:00 p.m.
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 2 days
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-70-8505

Order Name (ชื่อการทดสอบ):
ANA Profile II by Immunoblot

 
 
Clinical Information (ข้อมูลทางคลินิก):
Antibodies against nuclear antigens (ANA) are directed against various cell nuclear components (biochemical substances in the cell nucleus). These encompass nucleic acids, cell nuclear proteins and ribonucleoproteins. The ANA profile II used for detection autoantibodies against individual or several cell nuclear autoantigens is an essential element in the diagnosis of autoimmune diseases, particularly rheumatic diseases i.e. Anti-nRNP/Sm, Anti Sm, Anti SS-A, Anti Ro-52, Anti SS-B, Anti Scl-70, Anti PM-Scl, Anti Jo-1, Anti CENP B, Anti PCNA, Anti dsDNA, Anti Nucleosomes, Anti Histones, Anti Ribosomal P-Protein, Anti AMA-M2, Anti DFS70.
The investigation of ANA and subsequent differentiation within the ANA or ENA spectrum contributes greatly to establishing a diagnosis, particularly in the following rheumatic diseases:
- Systemic lupus erythematosus (SLE)
- Sharp syndrome (mixed connective tissue disease = MCTD)
- Sjögren’s syndrome
- Systemic sclerosis (systemic scleroderma, SSC)
- Limited form of systemic sclerosis (CREST syndrome)
- Myositis (poly-/dermatomyositis)
- Rheumatoid arthritis
- Overlap syndromes
- Primary biliary cholangitis
 
Reference Value (ค่าอ้างอิง):
Test: ANA Profile II
 
Tests Reference Value
Anti nRNP/Sm Negative
Anti Sm Negative
Anti SS-A Negative
Anti Ro-52 Negative
Anti SS-B Negative
Anti Scl-70 Negative
Anti Jo-1 Negative
Anti Centromere Protein B (CENP B) Negative
Anti PCNA Negative
Anti dsDNA Negative
Anti Nucleosomes Negative
Anti Histones Negative
Anti Ribosomal P-Protein Negative
Anti AMA-M2 Negative
Anti DFS70 Negative
 
Interpretation (การแปลผล):
The serological detection of autoantibodies against individual or several cell nuclear autoantigens is an essential element in the diagnosis of autoimmune diseases.  The frequency (prevalence) of anti-nuclear antibodies in inflammatory rheumatic diseases in between 20% and 100% (in rheumatoid arthritis between 20% and 40%).  Therefore, differential ANA diagnostics to detect autoantibodies against different nuclear antigens is indispensable for the identification of individual rheumatic diseases. The ANA profiles offer innovative test combination based on the lineblot technology.  Positive test resulst provide important serodiagnostic information for the diagnosis of the rheumatic diseases, as well as further autoimmune disease such as primary biliary liver cholangitis (PBC). However, antibodies against nuclear antigens are detectable in subjectively healthy individuals, usually at a low level.  This finding is more common in women than men and the frequency of a detectable ANA in healthy women over 40 years of age may approach 15% to 20%. ANA may also be detectable following viral illnesses, in chronic infections, or in patients treated with many different medications.  A diagnosis should not be made based on a single test result. The clinical symptoms of the patient should always be taken into account along with the serological results by the physician.  
 
Clinical Reference (เอกสารอ้างอิง):
Manufacturer’s reagent package insert, EUROLINE ANA Profile3 plus DFS70 (IgG), February 2019, EUROIMMUN, Lübeck, D-23560 Germany.