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Clear
 
Test Code (รหัสการทดสอบ):
090-40-0740-01

Order Name (ชื่อการทดสอบ):
AFB Stain (Source Specify)

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Tissue/ Sterile container or Microscopic slide
  4. Urine/ Sterile container
  5. Stool/ Sterile container
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 hours
 
Useful For (ประโยชน์การทดสอบ):
Detection of acid-fast bacilli in clinical specimens
 
Methodology (วิธีการทดสอบ):
Kinyoun stain
 
AliasesName (ชื่อเรียกอื่นๆ) :
Acid-Fast Bacilli (AFB)
AFB (Acid-Fast Bacilli)
Bacillus, Acid-Fast
MTB (Mycobacterium tuberculosis)
TB (Tuberculosis) 
 
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0740-01

Order Name (ชื่อการทดสอบ):
AFB Stain (Source Specify)

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Patient with clinical and chest x-ray findings compatible with tuberculosis should collect 3 samples at 8-24 hour intervals and should include at least one first morning specimen.
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Tissue/ Sterile container or Microscopic slide
  4. Urine/ Sterile container
  5. Stool/ Sterile container
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Sputum, Bronchoalveolar lavage, Bronchial wash volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue volume Entire collection (minimum 0.5 mL)
  4. Urine, volume Entire collection (minimum 1 mL)
  5. Stool, volume Entire collection (minimum 1 gram.)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Hemolysis : N/A
Lipemia : N/A
Icterus : N/A
Other : Swab in Stuart transport medium, Inadequate sputum will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash (BW) Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage (BAL) Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Sputum Refrigerated, 2oC to 8oC 24 hours
Stool Refrigerated, 2oC to 8oC 24 hours
Tissue Room temperature, 18oC to 25oC 24 hours
Urine Refrigerated, 2oC to 8oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0740-01

Order Name (ชื่อการทดสอบ):
AFB Stain (Source Specify)

 
Method detail (วิธีการทดสอบ):
Kinyoun stain
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Received specimen to reported within 3 hours
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
7 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0740-01

Order Name (ชื่อการทดสอบ):
AFB Stain (Source Specify)

 
 
Clinical Information (ข้อมูลทางคลินิก):
Mycobacterium tuberculosis is a leading infectious disease cause of death worldwide. The Centers for Disease Control and Prevention has reported a rise in the incidence of tuberculosis associated with AIDS, foreign-born cases, and increased transmission in high-risk populations. There has also been a rise in the number of       
M. tuberculosis strains that exhibit resistance to one or more antituberculosis drugs. The public health implications of these facts are considerable. Because M. tuberculosis is readily spread by airborne particles, rapid diagnosis and isolation of infected persons is important. Nontuberculous mycobacteria infections also cause significant morbidity and mortality in humans, particularly in immunocompromised persons. Detection of acid-fast bacilli in sputum specimens allows rapid identification of individuals who are likely to be infected with mycobacteria while definitive diagnosis and treatment are pursued.
 
Reference Value (ค่าอ้างอิง):
No AFB observed
 
Interpretation (การแปลผล):
Patients whose specimens are identified as acid-fast positive should be considered infected with Mycobacterium, should diagnosis by mycobacterial culture (AFB culture).
 
Clinical Reference (เอกสารอ้างอิง):
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. ​https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)