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Clear
 
Test Code:
090-40-0740-01

Order Name:
AFB Stain (Source Specify)

 
Useful For:
Detection of acid-fast bacilli in clinical specimens
 
Methodology:
Kinyoun stain
 
AliasesName:
Acid-Fast Bacilli (AFB)
AFB (Acid-Fast Bacilli)
Bacillus, Acid-Fast
MTB (Mycobacterium tuberculosis)
TB (Tuberculosis) 
 
 
 
 
Test Code:
090-40-0740-01

Order Name:
AFB Stain (Source Specify)

 
Patient Preparation:
Patient with clinical and chest x-ray findings compatible with tuberculosis should collect 3 samples at 8-24 hour intervals and should include at least one first morning specimen.
 
Collection Specimen Or Container:
  1. CSF, Body fluids/ Sterile container
  2. Sputum, Bronchoalveolar lavage, Bronchial wash/ Sterile container
  3. Wound, Pus, Abscess, Discharge, Tissue/ Sterile container or Microscopic slide
  4. Urine/ Sterile container
  5. Stool/ Sterile container
 
Specimen Testing Type:
  1. CSF, Body fluids, volume Entire collection (minimum 0.5 mL)
  2. Sputum, Bronchoalveolar lavage, Bronchial wash volume Entire collection (minimum 0.5 mL)
  3. Wound, Pus, Abscess, Discharge, Tissue volume Entire collection (minimum 0.5 mL)
  4. Urine, volume Entire collection (minimum 1 mL)
  5. Stool, volume Entire collection (minimum 1 gram.)
 
Sub Mission Container:
Sterile container or Apply original sample to surface of Microscopic slide using appropriate application method (determined by consistency of specimen type)
 
Rejection Criteria:
Hemolysis : N/A
Lipemia : N/A
Icterus : N/A
Other : Swab in Stuart transport medium, Inadequate sputum will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bronchial wash (BW) Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage (BAL) Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 24 hours
Discharge Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Sputum Refrigerated, 2oC to 8oC 24 hours
Stool Refrigerated, 2oC to 8oC 24 hours
Tissue Room temperature, 18oC to 25oC 24 hours
Urine Refrigerated, 2oC to 8oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0740-01

Order Name:
AFB Stain (Source Specify)

 
Method detail:
Kinyoun stain
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to reported within 3 hours.
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-40-0740-01

Order Name:
AFB Stain (Source Specify)

 
 
Clinical Information:
Mycobacterium tuberculosis is a leading infectious disease cause of death worldwide. The Centers for Disease Control and Prevention has reported a rise in the incidence of tuberculosis associated with AIDS, foreign-born cases, and increased transmission in high-risk populations. There has also been a rise in the number of       
M. tuberculosis strains that exhibit resistance to one or more antituberculosis drugs. The public health implications of these facts are considerable. Because M. tuberculosis is readily spread by airborne particles, rapid diagnosis and isolation of infected persons is important. Nontuberculous mycobacteria infections also cause significant morbidity and mortality in humans, particularly in immunocompromised persons. Detection of acid-fast bacilli in sputum specimens allows rapid identification of individuals who are likely to be infected with mycobacteria while definitive diagnosis and treatment are pursued.
 
Reference Value:
No AFB observed
 
Interpretation:
Patients whose specimens are identified as acid-fast positive should be considered infected with Mycobacterium, should diagnosis by mycobacterial culture (AFB culture).
 
Clinical Reference:
  1. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  2. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  3. ​https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)