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Test Code:
090-40-0980-01

Order Name:
AFB C/S (Source Specify)

 
Useful For:
Recovery of Mycobacterium spp. from infection specimens
 
Methodology:
  1. Continuously Monitored Automated broth Culture Instrument: BACTEC MGIT 320 (fluorescence detection technic)
  2. ​Identification and susceptiblity testing by referral lab
 
AliasesName:
Acid-Fast Bacilli (AFB)
AFB (Acid-Fast Bacilli)
Bacillus, Acid-Fast
Culture, TB (Tuberculosis)
MTB (Mycobacterium tuberculosis)
Mycobacteria Culture
Mycobacterium tuberculosis (MTB)
TB (Tuberculosis)
TB (Tuberculosis) Culture
 
 
 
Test Code:
090-40-0980-01

Order Name:
AFB C/S (Source Specify)

 
Patient Preparation:
Medications: Do not use antibiotics before collection of specimen.
 
Patient with clinical and chest x-ray findings compatible with tuberculosis should collect 3 samples at 8-24 hour intervals and should include at least one first morning specimen.
 
Collection Specimen Or Container:
  1. Blood/ BD BACTEC MYCO/F-lytic
  2. Bone marrow/ BD BACTEC MYCO/F-lytic
  3. CSF, Body fluid, fluid/ Sterile container
  4. Sputum, Bronchial wash, Bronchoalveolar lavage/ Sterile container
  5. Urine/ Sterile container
  6. Stool/ Sterile container
  7. Pus, wound, abscess, eye exudate, skin lesion, tissue, other specimen/ swab in Sterile container with normal saline 2-3 mL.
 
Specimen Testing Type:
  1. Blood, volume 3-5 mL. (minimum volume 1 mL.)
  2. Bone marrow, volume 3-5 mL. (minimum volume 1 mL.)
  3. CSF, Body fluid, volume Entire collection
  4. Sputum, Bronchial wash, Bronchoalveolar lavage, minimum volume 3 mL.
  5. Urine, minimum volume 10 mL.
  6. Stool, minimum volume 1 gram
  7. Pus, wound, abscess, eye exudate, skin lesion, tissue, other specimen, volume Entire collection
 
Sub Mission Container:
  1. BD BACTEC MYCO/F-lytic (Red top glass vial)
  2. Sterile container
  3. Swab in Sterile container with normal saline 2-3 mL.
 
Rejection Criteria:
Specimen in formalin or dry swab will be reject.
 
Specimen Stabillity:
Specimen Type Temperature Time
Abscess Room temperature, 18oC to 25oC 24 hours
Blood Room temperature, 18oC to 25oC 2 hours
Body fluid Room temperature, 18oC to 25oC 24 hours
Bone marrow Room temperature, 18oC to 25oC 2 hours
Bronchial wash (BW) Refrigerated, 2oC to 8oC 24 hours
Bronchoalveolar lavage (BAL) Refrigerated, 2oC to 8oC 24 hours
CSF Room temperature, 18oC to 25oC 15 mins
Eye exudate Room temperature, 18oC to 25oC 24 hours
Pus Room temperature, 18oC to 25oC 24 hours
Skin lesion Room temperature, 18oC to 25oC 24 hours
Stool Refrigerated, 2oC to 8oC 24 hours
Tissue Room temperature, 18oC to 25oC 24 hours
Urine Refrigerated, 2oC to 8oC 24 hours
Wound Room temperature, 18oC to 25oC 24 hours
Other specimen Room temperature, 18oC to 25oC 24 hours
 
 
 
Test Code:
090-40-0980-01

Order Name:
AFB C/S (Source Specify)

 
Method detail:
  1. Continuously Monitored Automated broth Culture Instrument: BACTEC MGIT 320 (fluorescence detection technic)
  2. Identification and susceptiblity testing by referral lab
 
Schedule:
Tested daily (24 hours)
 
Turnaround Time:
Received specimen to reported within 2 months
 
Performing Location:
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time:
  1. CSF for 1 month
  2. Other specimen for 7 days
 
 
 
Test Code:
090-40-0980-01

Order Name:
AFB C/S (Source Specify)

 
 
Clinical Information:
Mycobacterial disease is known to have affected humans for more than a century and still it continues to be a global health concern. There are several challenges as far as TB is concerned.

Mycobacteremia occurs most often in immunocompromised hosts. The majority of disseminated mycobacterial infections are due to Mycobacterium avium complex but bacteremia can also be caused by other mycobacterial species including, but not limited to, Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium fortuitum, Mycobacterium chelonae, Mycobacterium scrofulaceum, Mycobacterium szulgai, and Mycobacterium xenopi.

Mycobacterial blood cultures may be indicated for patients presenting with signs and symptoms of sepsis, especially fever of unknown origin.

Nontuberculous mycobacteria are a type of bacteria found in water and soil. These bacteria are typically harmless. However, when they enter the body, they can cause skin lesions, soft tissue infections, and serious lung problems.
 
Reference Value:
No Mycobacterium spp. isolated
 
Interpretation:
  1. Positive AFB cultures will send to referral laboratory for organism identification and susceptiblity testing.
  2. Negative reports are issued after 42 days incubation.
 
Clinical Reference:
  1. Manufacturer’s Package Insert, BD BBLTM MGITTM Mycobacteria Growth Indicator tube 7mL with BACTECTM MGITTM 960 Supplement kit, L000180JAA, 2013/07, Becton, Dickinson and Company, USA.
  2. https://www.mayocliniclabs.com (Retrieved: 29 Jan 2019)
  3. https://nyulangone.org/conditions/nontuberculous-mycobacterial-infections/diagnosis