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Clear
 
Test Code:
090-70-1411

Order Name:
Respiratory pathogens 33 (RP33), Source specify

 
Useful For:
The test is indicated as an aid in the detection and identification of viruses, bacteria and fungi causing respiratory tract infections in symptomatic adult and pediatric patients
 
Methodology:
Multiplex Reverse-Transcriptase Polymerase Chain Reaction 
 
Test List In Profile:
Influenza A
Influenza A (H1N1)
Influenza B
Influenza C
Rhinovirus
Coronavirus 229E
Coronavirus NL63
Coronavirus HKU1
Coronavirus OC43
Parainfluenza 1
Parainfluenza 2
Parainfluenza 3
Parainfluenza 4
Metapneumovirus A/B
Bocavirus
Respiratory syncytial virus A/B
Parechovirus
Enterovirus
Adenovirus
Mycoplasma pneumoniae
Chlamydophila pneumoniae
Legionella pneumophila/Legionella longbeachae
Staphylococcus aureus
Streptococcus pneumoniae
Haemophilus Influenzae
Haemophilus Influenzae
Type B
Klebsiella pneumoniae
Salmonella
spp.
Moraxella catarrhalis
Pneumocystis jirovecii
Bordetella
 spp. except Bordetella parapertussis
 
AliasesName:
Respiratory pathogen panel (RPP)
Respiratory viral panel (RVP)
Respiratory 33 pathogens panel
PCR for Respiratory Pathogen 33
Respiratory Pathogen Panel 33 (RP33)
 
 
 
Test Code:
090-70-1411

Order Name:
Respiratory pathogens 33 (RP33), Source specify

 
Collection Specimen Or Container:
  1. Throat swab/Universal Transport Media (UTM) 3 mL, 1 tube or
  2. Bronchoalveolar lavage (BAL)/Sterile Container or
  3. Bronchial washing/Sterile Container or
  4. Sputum/Sterile Container
 
Specimen Testing Type:
  1. Throat swab/ Universal Transport Media (UTM) 3 mL, 1 tube
  2. Bronchoalveolar lavage (BAL), minimum volume 1.5 mL
  3. Bronchial washing, minimum volume 1.5 mL
  4. Sputum, minimum volume 0.5 mL
 
Sub Mission Container:
Universal Transport Media (UTM) tube or
Sterile Container 
 
Specimen Stabillity:
Specimen Type Temperature Time
Throat swab Refrigerated, 2oC to 8oC 7 days
Bronchoalveolar lavage (BAL)
Bronchial washing
Sputum
 
 
 
Test Code:
090-70-1411

Order Name:
Respiratory pathogens 33 (RP33), Source specify

 
Method detail:
Multiplex Reverse-Transcriptase Polymerase Chain Reaction 
 
Schedule:
Test daily, at 10:00 a.m.
 
Turnaround Time:
Received specimen to reported within 1 days.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department
Tel.14168
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-70-1411

Order Name:
Respiratory pathogens 33 (RP33), Source specify

 
 
Clinical Information:
Respiratory viruses, bacteria and fungi can cause the lower respiratory tract infection (LRTI) that range in severity, and have the potential to cause severe disease especially in children, the elderly, and immunocompromised individuals.  Lower respiratory symptoms can include shortness of breath, weakness, high fever, coughing, and fatigue. Due to the similarity of diseases caused by many viruses and bacteria, diagnosis based on clinical symptoms alone is difficult. About 90% of the LRTI cases are caused by different viruses, such as adenovirus, coronavirus, parainfluenza, influenza, and rhinovirus and less than 10% to bacteria, such as Bordetella pertussis, Chlamydophila pneumoniae, and Mycoplasma pneumoniae. However, antibiotics are often prescribed for respiratory infections despite the viral etiology in 90% of cases. Clarity on the causative pathogen in respiratory illness aids patient diagnosis and treatment management and can help to reduce over prescribing of antibiotics. 
 
Reference Value:
Parameter Reference value
Influenza A Negative
Influenza A (H1N1) Negative
Influenza B   Negative
Influenza C Negative
Rhinovirus Negative
Coronavirus 229E Negative
Coronavirus NL63 Negative
Coronavirus HKU1 Negative
Coronavirus OC43 Negative
Parainfluenza 1 Negative
Parainfluenza 2 Negative
Parainfluenza 3 Negative
Parainfluenza 4 Negative
Metapneumovirus A/B Negative
Bocavirus Negative
Respiratory syncytial virus A/B Negative
Parechovirus Negative
Enterovirus Negative
Adenovirus Negative
Mycoplasma pneumoniae Negative
Chlamydophila pneumoniae Negative
Legionella pneumophila/Legionella longbeachae Negative
Staphylococcus aureus   Negative
Streptococcus pneumoniae Negative
Haemophilus Influenzae Negative
Haemophilus Influenzae Type B Negative
Klebsiella pneumoniae Negative
Salmonella spp.     Negative
Moraxella catarrhalis Negative
Pneumocystis jirovecii Negative
Bordetella  spp. except Bordetella parapertussis Negative
 
Interpretation:
This test may not be able to differentiate newly emerging Influenza A HA subtypes.  The detection of pathogen nucleic acids is dependent upon proper specimen collection, handling, transportation, storage and preparation (including extraction). There is a risk of false negative values resulting from improperly collected, transported, or handled specimens. Negative results do not preclude influenza virus infection and should not be used as the sole basis for treatment or other patient management decisions. Positive results do not rule out co-infection with other pathogens. Detection of bacterial nucleic acid may be indicative of colonizing or normal respiratory flora and may not indicate the causative agent of pneumonia. The agent detected may not be the definite cause of disease. The use of additional laboratory testing (e.g. bacterial and viral culture, immunofluorescence, and radiography) and clinical presentation must be taken into consideration in order to obtain the final diagnosis of respiratory tract infection.
 
Clinical Reference:
Manufacturer’s package inserts. FTD Respiratory pathogens 33, February 2018, Fast Track Diagnostics, rue Henri Koch, L4354 Esch-sur-Alzette, Luxembourg.