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Clear
 
Test Code:
090-70-0040-01

Order Name:
Plasma EBV (Viral load)

 
Useful For:
This test is used to measure the EBV viral load in whole blood. The viral load represents the number of virions and/or the number of EBV-infected B lymphocytes in the replication phase. Combined with other biological investigation methods (medical examination, biochemical and immunological analysis, etc.) the EBV viral load measurement is used to define a therapeutic strategy and monitor its efficacy. In certain cases, the EBV viral load is also useful to confirm an EBV primary infection, or to diagnose Hodgkin’s lymphoma or nasopharyngealcarcinoma.
 
Methodology:
Real Time Polymerase chain reaction (Real-Time PCR)
 
Test List In Profile:
Plasma EBV (Viral load)
Log equivalence
 
AliasesName:
EBV DNA level
EBV DNA PCR
EBV DNA quant
EBV PCR
EBV quant
EBV viral load
Epstein-Barr virus
Infectious mononucleosis
 
 
 
Test Code:
090-70-0040-01

Order Name:
Plasma EBV (Viral load)

 
Collection Specimen Or Container:
Blood/ K3 EDTA (Lavender Top) 3 mL, 2 tubes
 
Specimen Testing Type:
Plasma EDTA, minimum volume 1.5 mL
 
Sub Mission Container:
Plastic tube
 
Rejection Criteria:
Hemolysis: N/A
Lipemia: N/A
Icterus: N/A
Other: Heparin, anticoagulant blood
 
Specimen Stabillity:
Specimen Type Temperature Time
Whole blood Room temperature, 18oC to 25oC 24 hours
Plasma Refrigerated, 2oC to 8oC 1 week
 
 
 
Test Code:
090-70-0040-01

Order Name:
Plasma EBV (Viral load)

 
Method detail:
Real Time Polymerase chain reaction (Real-Time PCR)
 
Schedule:
Monday and Thursday, at 10:00 a.m.
 
Turnaround Time:
Received specimen to reported within 4 days.
 
Performing Location:
Molecular biology & Genetics, Laboratory Department Tel.14168
 
Specimen Retention Time:
7 days
 
 
 
Test Code:
090-70-0040-01

Order Name:
Plasma EBV (Viral load)

 
 
Clinical Information:
Epstein-Barr virus (EBV) is a ubiquitous herpesvirus that infects the majority of the world population (seroprevalence is greater than 95%). After a primary infection (transmission through contact with saliva), EBV remains latent in B cells. Periodically, EBV can reactivate in immunocompetent individuals. This reactivation is characterized by the production of virions in the oropharynx but is asymptomatic. EBV is the causative agent of infectious mononucleosis. It is also involved in the pathogenesis of several cancers such as Burkitt’s lymphoma, Hodgkin’s lymphoma, and nasopharyngeal carcinoma. In immunosuppressed individuals, EBV can lead to B-cell lymphoproliferative disorders. Indeed, the main characteristic of EBV is to cause the B lymphocytes to multiply and subsequently produce a malignant lymphoma. In this context, the measurement of the EBV viral load is particularly significant.
 
Reference Value:
Test Reference Value
Plasma EBV (Viral load) Not detected
Log equivalence Not detected
 
Interpretation:
  1. The quantification range of this assay is 150 to 1,500,000 copies/mL (2.18 log to 6.18 log copies/mL).
  2. An "Not detected" result indicates that Epstein-Barr virus (EBV) DNA was not detected in the plasma specimen.
  3. A result of "<150 copies/mL (<2.18 log copies/mL)" indicates that EBV DNA is detected, but the EBV DNA level present cannot be quantified accurately below this lower limit of quantification of this assay. When clinically indicated, follow-up testing with this assay is recommended in 1 to 2 months.
  4. Two quantification results are considered as significantly different if the two values expressed in log10 (copies/mL of sample) differ by at least 0.5 log10. This interpretation only applies if the 2 quantification results were obtained using the same PCR real time instrument and the same extraction methods.
  5. It is absolutely necessary to interprete the test results include with other clinical and biological test results in order to define patient viral status.
 
Clinical Reference:
Manufacturer’s package insert, artus® EBV QS-RGQ Kit Handbook, October 2014, Qiagen GmbH, D-40724 Hilden, Germany.