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Clear
 
Test Code (รหัสการทดสอบ):
090-40-0310-01

Order Name (ชื่อการทดสอบ):
Hemo C/S

 
Specimen / Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Bactec Plus Aerobic/F Vial (for adult)
Blood/ Bactec Ped’s Plus Vial (for pediatric)
 
Turnaround Time (ระยะเวลารอผล):
Preliminary Report:
     - Received to Preliminary Reported within 2 days. (48 hours)

Final Report:
     - Received to Final Reported within 5 days.
 
Useful For (ประโยชน์การทดสอบ):
Bacterial detection for infections of Septicemia (Blood)
 
Methodology (วิธีการทดสอบ):
  1. Continuously Monitored Blood culture Automated Instrument : BD BactecTM FX (fluorescence detection technic)
  2. Conventional Culture Technique for Hemoculture positive
  3. Identification by MALDI-TOF
  4. Suceptibility test by Vitek 2 Compact
 
AliasesName (ชื่อเรียกอื่นๆ) :
Aerobic Bacteria Culture,
Blood Bacteria,
Blood Culture,
Blood Septicemia
Sepsis
Bacteremia
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0310-01

Order Name (ชื่อการทดสอบ):
Hemo C/S

 
Patient Preparation (การเตรียมตัวผู้ป่วย):
Medications: Do not use antibiotics before collection of specimen that cause sepsis.
 
Collection Specimen Or Container (สิ่งส่งตรวจ/ภาชนะ):
Blood/ Bactec Plus Aerobic/F Vial (for adult)
Blood/ Bactec Ped’s Plus Vial (for pediatric)
 
Specimen Testing Type (สิ่งส่งตรวจที่ใช้ในการทดสอบ):
Blood, Bactec Plus Aerobic/F Vial (for adult) volume 8-10 mL (minimum volume 3 mL)
Blood, Bactec Ped’s Plus Vial (for pediatric) volume 1-3 mL (minimum volume 0.5 mL)
 
Sub Mission Container (ภาชนะส่งตรวจ):
Bactec Plus Aerobic/F Vial (for adult) ; Gray top, plastic vial
Bactec Ped’s Plus Vial (for pediatric) ; Pink top, plastic vial
 
Rejection Criteria (เกณฑ์ปฏิเสธสิ่งส่งตรวจ):
Refrigerated specimen will be reject.
 
Specimen Stabillity (ความคงตัวของสิ่งส่งตรวจ):
Specimen Type Temperature Time
Blood Room temperature, 18oC to 25oC
2 hours
(Maximum 48 hours)
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0310-01

Order Name (ชื่อการทดสอบ):
Hemo C/S

 
Method detail (วิธีการทดสอบ):
  1. Continuously Monitored Blood culture Automated Instrument : BD BactecTM FX (fluorescence detection technic)
  2. Conventional Culture Technique for Hemoculture positive
  3. Identification by MALDI-TOF
  4. Suceptibility test by Vitek 2 Compact
 
Schedule (ตารางการทดสอบ):
Tested daily (24 hours)
 
Turnaround Time (ระยะเวลารอผล):
Preliminary Report:
     - Received to Preliminary Reported within 2 days. (48 hours)

Final Report:
     - Received to Final Reported within 5 days.
 
Performing Location (หน่วยงานที่ทำการทดสอบ):
Microbiology, Laboratory Department Tel. 14171
 
Specimen Retention Time (ระยะเวลาเก็บสิ่งส่งตรวจ):
5 days
 
 
 
Test Code (รหัสการทดสอบ):
090-40-0310-01

Order Name (ชื่อการทดสอบ):
Hemo C/S

 
 
Clinical Information (ข้อมูลทางคลินิก):
The detection of microorganisms in a patient’s blood has diagnostic and prognostic. importance. Blood cultures are essential in the diagnosis and treatment of the etiologic agents of sepsis. Bacterial sepsis constitutes one of the most serious infectious diseases and, therefore, the expeditious detection and identification of bloodborne bacterial pathogens is an important function of the diagnostic microbiology laboratory.
 
Reference Value (ค่าอ้างอิง):
No growth after 5 days
 
Interpretation (การแปลผล):
Positive cultures of Bacteria and yeast are reported with the organism identification and susceptiblity testing. Positive cultures are usually an indication of infection and are reported as soon as detected. Correlation of culture results and the clinical situation is required for optimal patient management. A final negative report is issued after 5 days of incubation.
 
Clinical Reference (เอกสารอ้างอิง):
  1. Manufacturer’s Reagent package insert, BD BactecTM FX
  2. Amy L. Leber, editor. Clinical Microbiology Procedure Handbook 4th Edition. Washington DC: American Society for Microbiology; 2016
  3. James H. Jorgensen, Michael A. PFALLER. Manual of Clinical Microbiology 11th ed. Washington, DC: ASM Press; 2015
  4. https://en.wikipedia.org/wiki/Bacteremia (Retrieved: 29 Jan 2019)