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Test Code:
090-71-5563

Order Name:
APOE genotype for Alzheimer’s disease (Alleles E2, E3 and E4) **

 
Useful For:
Apolipoprotein E (ApoE) plays an important role in the catabolism of lipoproteins and cholesterol. Three major alleles of ApoE are known: ApoE3 (112C 158R, normal function), ApoE2 (112C 158C, Type III Hyperlipidemia), ApoE4 (112R 158R, increased cholesterol levels1). Homozygote ApoE4 individuals (112R/R 158R/R) have en elevated risk for development of the Alzheimer disease.
 
Methodology:
Multiplex Tetraprimers-ARMS PCR
 
Test List In Profile:
APOE Alleles E2, E3 and E4
 
AliasesName:
Apolipoprotein E genotyping
 
 
 
Test Code:
090-71-5563

Order Name:
APOE genotype for Alzheimer’s disease (Alleles E2, E3 and E4) **

 
Patient Preparation:
N/A
 
Collection Specimen Or Container:
Blood/ EDTA (Lavender top) 3 mL, 2 tubes
 
Specimen Testing Type:
EDTA Whole Blood, minimum volume 3-5 mL
 
Sub Mission Container:
Original tube
 
 
 
Test Code:
090-71-5563

Order Name:
APOE genotype for Alzheimer’s disease (Alleles E2, E3 and E4) **

 
Method detail:
Multiplex Tetraprimers-ARMS PCR for codon 112 and 158 of ApoE gene
 
Schedule:
N/A **Sent Out to N Health
 
Turnaround Time:
Received specimen to report within 10-14 days
 
Performing Location:
N Health
Referral Lab Services, Laboratory Department 14160-2
 
 
 
Test Code:
090-71-5563

Order Name:
APOE genotype for Alzheimer’s disease (Alleles E2, E3 and E4) **

 
 
Clinical Information:
Apolipoprotein E (ApoE) plays an important role in the catabolism of lipoproteins and cholesterol.
Three major alleles of ApoE are known; E2 (Cys112/Cys158) (, E3 (Wild-type; Cys112/Arg158) and E4 (Arg158/Arg158.

A meta-analysis of clinical and autopsy-based studies demonstrated that, in white individuals, risk of AD was
increased in individuals with one copy of the ε4 allele (E2/E4, OR 2.6; E3/E4, OR 3.2) or two copies (E4/E4, OR 14.9) compared with those with an E3/E3 genotype (Wild-type).

Conversely, the E2 allele of APOE has a protective effect against AD: the risk of AD in individuals carrying
APOE E2/E2 (OR 0.6) or E2/E3 (OR 0.6) is lower than in those carrying E3/E3 (Wild-type).

APOE E4–AD association was weaker among African American (E4/E4, OR 5.7) and Hispanic (E4/E4, OR 2.2)
populations, and was stronger in Japanese people (E4/E4, OR 33.1) compared with white individuals (E4/E4, OR 12.5). APOE E4 is associated with increased prevalence of AD and lower age of onset. The frequency of AD and mean age at clinicalonset are 91% and 68 years in E4 homozygotes, 47% and 76 years in E4 heterozygotes, and 20% and 84 years in E4 noncarriers indicating that APOE E4 confers dramatically increased risk of development of AD with an earlier age of onset in a gene dose-dependent manner.

Sensitivity : These reagents detect 1 ng of human genomic DNA.
Specificity : The allele specific primers were designed specifically to four alleles; Cys112, Cys158, Arg112 and Arg158.
 
 
Reference Value:
Wild-type of apolipoprotein is E3/E3.
 
Clinical Reference:
N Health Laboratory Catalog